Human Haploid Cells Differentiated from Meiotic Competent Clonal Germ Cell Lines That Originated from Embryonic Stem Cells
| Autor: | Amalia Gallegos-Cardenas, Jennifer Mumaw, Steven L. Stice, Amber Young, Franklin D. West |
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| Rok vydání: | 2011 |
| Předmět: |
Cellular differentiation
Cell Separation Haploidy Biology Mice Meiosis Cell Movement medicine Animals Humans Cells Cultured Embryonic Stem Cells Cell Proliferation Regulation of gene expression Genetics Cell growth Cell Differentiation Cell Biology Hematology Phenotype Embryonic stem cell Clone Cells Cell biology Germ Cells medicine.anatomical_structure Gene Expression Regulation Ploidy Biomarkers Germ cell Developmental Biology |
| Zdroj: | Stem Cells and Development. 20:1079-1088 |
| ISSN: | 1557-8534 1547-3287 |
| Popis: | Early germ-like cells (GLCs) derived from human embryonic stem cells (hESCs) have presented new opportunities to study germ cell differentiation in vitro. However, differentiation conditions that facilitate the formation of haploid cells from the derived GLCs have eluded the field. The inability to propagate GLCs in culture is a further limitation, resulting in inconsistent rederivations of GLCs from hESCs with relatively few GLCs in these heterogeneous populations. Here we found in vitro conditions that enrich for DDX4/POU5F1+ GLCs (∼60%) and that has enabled continual propagation for50 passages without loss of phenotype. Clonal isolation of single GLCs from these mixed cultures generated 3 GLC (90% DDX4/POU5F1+) and 2 hESC (0.1% DDX4+) lines that could be continually expanded without loss of phenotype. Differentiation of clonal GLC lines in serum resulted in expression of postmeiotic markers and11% were haploid, ∼5-fold higher than previous studies. The robust clonal meiotic competent and incompetent GLC lines will be used to understand the factors controlling human germ cell meiosis and postmeiotic maturation. |
| Databáze: | OpenAIRE |
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