Long non-coding RNA CRNDE regulates the growth and migration of prostate cancer cells by targeting microRNA-146a-5p
| Autor: | Chenghui Fan, Li'e Zang, Tongyi Men, Huamao Jiang, Dewang Fu, Zhaowei Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
lncrna crnde Down-Regulation Apoptosis Bioengineering Applied Microbiology and Biotechnology Flow cytometry Downregulation and upregulation Cell Movement Cell Line Tumor medicine Humans Neoplasm Invasiveness RNA Small Interfering Cell Proliferation Gene knockdown Base Sequence medicine.diagnostic_test Chemistry Cell growth Prostatic Neoplasms Cancer General Medicine medicine.disease prostate cancer mir-146a-5p Long non-coding RNA Gene Expression Regulation Neoplastic Reverse transcription polymerase chain reaction MicroRNAs Matrix Metalloproteinase 9 Gene Knockdown Techniques Cancer research Matrix Metalloproteinase 2 RNA Long Noncoding TP248.13-248.65 Research Article Research Paper Biotechnology |
| Zdroj: | Bioengineered, Vol 12, Iss 1, Pp 2469-2479 (2021) Bioengineered article-version (VoR) Version of Record |
| ISSN: | 2165-5987 2165-5979 |
| Popis: | The function of lncRNA CRNDE and its role in prostate cancer (PC) remains unclear. The aim of this study was to determine the expression level of lncRNA CRNDE in PC tissues and to elucidate its role in PC. The expression levels of lncRNA CRNDE were measured by quantitative reverse transcription polymerase chain reaction. The role of lncRNA CRNDE in PC cells was studied using loss-of-function assays in vitro. Cell proliferation, migration, invasion, and apoptosis were assessed via Cell Counting Kit-8, colony formation, flow cytometry, wound healing, and transwell chamber assays. A luciferase reporter assay was used to characterize the interaction between lncRNA CRNDE and miR-146a-5p. In PC tissues, the expression level of lncRNA CRNDE was upregulated. Moreover, knockdown of lncRNA CRNDE suppressed PC cell proliferation and migration and induced apoptosis in vitro. miR-146a-5p was verified as a direct target of lncRNA CRNDE. Moreover, the inhibition of miR-146a-5p partially counteracted the effects of lncRNA CRNDE on PC cell proliferation, migration, and invasion. In conclusion, lncRNA CRNDE may serve as a cancer promoter in PC by targeting miR-146a-5p. Therefore, lncRNA CRNDE could be a promising target for the clinical treatment of PC. Graphical abstract |
| Databáze: | OpenAIRE |
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