Knockdown of Long Noncoding RNA NR_026689 Inhibits Proliferation and Invasion and Increases Apoptosis in Ovarian Carcinoma HO-8910PM Cells
| Autor: | Yun-Ping Zhang, Xin Zhang, Xiuying Xie, Shao-Wen Li, Chun-Li Dong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Proliferation Caspase 3 Apoptosis Biology Article Metastasis 03 medical and health sciences 0302 clinical medicine Ovarian cancer Ovarian carcinoma NR_026689 Cell Line Tumor medicine Humans Neoplasm Invasiveness Cell Proliferation Ovarian Neoplasms Cell migration General Medicine medicine.disease Molecular biology 030104 developmental biology medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis Gene Knockdown Techniques Female RNA Long Noncoding |
| Zdroj: | Oncology Research |
| ISSN: | 1555-3906 0965-0407 |
| Popis: | Ovarian cancer is one of the leading causes of gynecological cancer-related deaths worldwide. We investigated the role of a newly discovered long noncoding RNA, NR_026689, in cell proliferation, metastasis, and apoptosis in ovarian cancer cells. Our results showed that NR_026689 was overexpressed in both clinical ovarian cancer patients and cultured ovarian cancer cells. Knockdown of NR_026689 in HO-8910PM cells significantly decreased the cell proliferative rate and the ability to form colonies. Transwell assays revealed that depletion of NR_026689 suppressed cell migration ability by 68% and cell invasive capacity by 71% in HO-8910PM cells. Moreover, specific shRNAs against NR_026689 notably promoted cell apoptosis in HO-8910PM cells by upregulating the expression of proapoptotic proteins, including caspase 3, caspase 9, cytochrome C, and PARP. Our study suggests an oncogenic potential of NR_026689 in ovarian cancer and might provide novel clues for the diagnosis and treatment of ovarian cancer in the clinic. |
| Databáze: | OpenAIRE |
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