Substrate Micropatterning as a New in Vitro Cell Culture System to Study Myelination
| Autor: | Dalinda Liazoghli, Peter Thostrup, Alejandro D. Roth, David R. Colman |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Letter
Neurite Physiology Cognitive Neuroscience Central nervous system oligodendrocytes 02 engineering and technology Biology Biochemistry Myelination 03 medical and health sciences Compact myelin medicine Schwann cells dorsal root ganglia neurons in vitro cell culture system Process (anatomy) 030304 developmental biology 0303 health sciences Matrigel Cell Biology General Medicine 021001 nanoscience & nanotechnology micropatterning medicine.anatomical_structure nervous system Peripheral nervous system 0210 nano-technology NODAL Neuroscience Micropatterning |
| Zdroj: | ACS Chemical Neuroscience ACS CHEMICAL NEUROSCIENCE Artículos CONICYT CONICYT Chile instacron:CONICYT |
| ISSN: | 1948-7193 |
| DOI: | 10.1021/cn2000734 |
| Popis: | Myelination is a highly regulated developmental process whereby oligodendrocytes in the central nervous system and Schwann cells in the peripheral nervous system ensheathe axons with a multilayered concentric membrane. Axonal myelination increases the velocity of nerve impulse propagation. In this work, we present a novel in vitro system for coculturing primary dorsal root ganglia neurons along with myelinating cells on a highly restrictive and micropatterned substrate. In this new coculture system, neurons survive for several weeks, extending long axons on defined Matrigel tracks. On these axons, myelinating cells can achieve robust myelination, as demonstrated by the distribution of compact myelin and nodal markers. Under these conditions, neurites and associated myelinating cells are easily accessible for studies on the mechanisms of myelin formation and on the effects of axonal damage on the myelin sheath. |
| Databáze: | OpenAIRE |
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