Time-resolved analysis of Staphylococcus aureus invading the endothelial barrier
| Autor: | Harita Yedavally, Anna Salvati, Elisa J M Raineri, Jan Maarten van Dijl |
|---|---|
| Přispěvatelé: | University of Groningen, Nanomedicine & Drug Targeting, Biopharmaceuticals, Discovery, Design and Delivery (BDDD), Nanotechnology and Biophysics in Medicine (NANOBIOMED), Microbes in Health and Disease (MHD), Translational Immunology Groningen (TRIGR) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
staphylococcus aureus
Microbiology (medical) endothelium Endothelium media_common.quotation_subject Cells Immunology Infectious and parasitic diseases RC109-216 Methicillin-Resistant Staphylococcus aureus/pathogenicity Microscopy Electron/methods Umbilical Veins/cytology medicine.disease_cause Microbiology Staphylococcus aureus/classification Flow cytometry 03 medical and health sciences medicine Humans Cytoplasm/microbiology Internalization Pathogen Cells Cultured 030304 developmental biology media_common 0303 health sciences Microscopy Cultured biology medicine.diagnostic_test 030306 microbiology Electron/methods invasion biology.organism_classification intracellular Flow Cytometry/methods 3. Good health Endothelial stem cell Infectious Diseases medicine.anatomical_structure Staphylococcus aureus Host-Pathogen Interactions mrsa Parasitology Endothelial Cells/microbiology Intracellular Bacteria |
| Zdroj: | Virulence, 11(1), 1623-1639. Taylor & Francis Group Virulence, Vol 11, Iss 1, Pp 1623-1639 (2020) Virulence |
| ISSN: | 2150-5594 |
| Popis: | Staphylococcus aureus is a leading cause of infections world-wide. Once this pathogen has reached the bloodstream, it can invade different parts of the human body by crossing the endothelial barrier. Infected endothelial cells may be lysed by bacterial products, but the bacteria may also persist intracellularly, where they are difficult to eradicate with antibiotics and cause relapses of infection. Our present study was aimed at investigating the fate of methicillin resistant S. aureus (MRSA) isolates of the USA300 lineage with different epidemiological origin inside endothelial cells. To this end, we established two in vitro infection models based on primary human umbilical vein endothelial cells (HUVEC), which mimic conditions of the endothelium when infection occurs. For comparison, the laboratory strain S. aureus HG001 was used. As shown by flow cytometry and fluorescence- or electron microscopy, differentiation of HUVEC into a cell barrier with cell-cell junctions sets limits to the rates of bacterial internalization, the numbers of internalized bacteria, the percentage of infected cells, and long-term intracellular bacterial survival. Clear strain-specific differences were observed with the HG001 strain infecting the highest numbers of HUVEC and displaying the longest intracellular persistence, whereas the MRSA strains reproduced faster intracellularly. Nonetheless, all internalized bacteria remained confined in membrane-enclosed LAMP-1-positive lysosomal or vacuolar compartments. Once internalized, the bacteria had a higher propensity to persist within the differentiated endothelial cell barrier, probably because internalization of lower numbers of bacteria was less toxic. Altogether, our findings imply that intact endothelial barriers are more likely to sustain persistent intracellular infection. |
| Databáze: | OpenAIRE |
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