Prostate — Specific G Protein Couple Receptor Genes and STAG1/PMEPA1 in Peripheral Blood from Patients with Prostatic Cancer
Autor: | M R Cardillo, Di Silverio F |
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Rok vydání: | 2006 |
Předmět: |
Male
Pathology medicine.medical_specialty medicine.drug_class Immunology urologic and male genital diseases Receptors G-Protein-Coupled 03 medical and health sciences Prostate cancer 0302 clinical medicine Prostate LNCaP medicine Humans Immunology and Allergy Receptor DNA Primers Pharmacology Messenger RNA Base Sequence Reverse Transcriptase Polymerase Chain Reaction business.industry Membrane Proteins Prostatic Neoplasms Cancer Hyperplasia Androgen medicine.disease medicine.anatomical_structure Case-Control Studies 030220 oncology & carcinogenesis business 030215 immunology |
Zdroj: | International Journal of Immunopathology and Pharmacology. 19:871-878 |
ISSN: | 2058-7384 |
Popis: | We investigated whether prostate - specific G protein couple receptor genes (PSGR) and STAG1/PMEPA1 gene expression in peripheral- blood could be useful as a diagnostic or prognostic marker of prostate cancer. Circulating cells were identified by reverse transcription-polymerase chain reaction (RT-PCR) to detect PSGR and STAG1/PMEPA1 mRNA in peripheral blood (PB) from 11 patients with treated prostate cancer (CaP), 11 with newly-diagnosed untreated CaP and 20 with benign prostatic hyperplasia (BPH) (controls). RT-PCR amplified PSGR in 8 of 11 untreated and in 9 of 11 treated patients with CaP and in 16 of 20 with BPH; whereas it amplified PMEPA1 in 1 of 11 untreated and in 7 of 11 treated patients with CaP and in 4 of 20 with BPH. In our control tissues and cell lines nearly all the prostatic and non- prostatic tissues and cell lines expressed PSGR mRNA, whereas only one prostatic neoplastic tissue and the androgen-responsive (LNCaP) and androgen non-responsive (PC3) prostatic cell lines expressed PMEPA1. These findings suggest that the investigated genes are poorly specific and probably of little use as diagnostic or prognostic markers in peripheral blood for monitoring prostate cancer progression and recurrence. |
Databáze: | OpenAIRE |
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