A Role for COX20 in Tolerance to Oxidative Stress and Programmed Cell Death in Saccharomyces cerevisiae
| Autor: | Gregory A. Tucker, Chenyu Du, Ethiraju R. Keerthiraju, Darren Greetham |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Programmed cell death COX20 Saccharomyces cerevisiae yeast medicine.disease_cause Microbiology Article 03 medical and health sciences Virology medicine Cytochrome c oxidase oxidative stress lcsh:QH301-705.5 programmed cell death 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species biology 030306 microbiology respiratory growth biology.organism_classification Yeast 3. Good health Cell biology lcsh:Biology (General) chemistry Apoptosis biology.protein Fermentation Oxidative stress |
| Zdroj: | Microorganisms Volume 7 Issue 11 Microorganisms, Vol 7, Iss 11, p 575 (2019) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms7110575 |
| Popis: | Industrial production of bioethanol from lignocellulosic materials (LCM&prime s) is reliant on a microorganism being tolerant to the stresses inherent to fermentation. Previous work has highlighted the importance of a cytochrome oxidase chaperone gene (COX20) in improving yeast tolerance to acetic acid, a common inhibitory compound produced during pre-treatment of LCM&rsquo s. The presence of acetic acid has been shown to induce oxidative stress and programmed cell death, so the role of COX20 in oxidative stress was determined. Analysis using flow cytometry revealed that COX20 expression was associated with reduced levels of reactive oxygen species (ROS) in hydrogen peroxide and metal-induced stress, and there was a reduction in apoptotic and necrotic cells when compared with a strain without COX20. Results on the functionality of COX20 have revealed that overexpression of COX20 induced respiratory growth in &Delta imp1 and &Delta cox18, two genes whose presence is essential for yeast respiratory growth. COX20 also has a role in protecting the yeast cell against programmed cell death. |
| Databáze: | OpenAIRE |
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