Modulation of interleukin-1 receptor expression by transforming growth factor-β in cultured rabbit articular chondrocytes: analysis by reverse transcription-polymerase chain reaction
| Autor: | N. Segond, Hubert Penfornis, Françoise Rédini, Margaret Macro, M.S. Moukhtar, Pronost S, Pujol Jp, Annick Jullienne |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Cartilage
Articular Interleukin-1 receptor type I Molecular Sequence Data Biomedical Engineering Down-Regulation Gene Expression Chick Embryo Rabbit articular chondrocytes Interleukin-1 receptor Biology Polymerase Chain Reaction Mice Species Specificity Rheumatology Transforming Growth Factor beta Complementary DNA Animals Humans Orthopedics and Sports Medicine RNA Messenger Receptor Gene Incubation Cells Cultured Base Composition Wound Healing Reverse transcription-polymerase chain reaction Base Sequence Transforming growth factor-β Receptors Interleukin-1 Molecular biology Reverse transcription polymerase chain reaction Rabbits Interleukin 1 receptor type I Transforming growth factor |
| Zdroj: | Osteoarthritis and Cartilage. 3:147-155 |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/s1063-4584(05)80049-4 |
| Popis: | Interleukin-1 receptor type I (IL-1RI) expression in cultured rabbit articular chondrocytes (RAC) was studied by reverse transcription-polymerase chain reaction (RT-PCR). A cDNA probe specific for the rabbit IL-1RI gene was constructed using primers derived from the sequence data of the human, murine and chick receptors. Transforming growth factor-beta 1 (TGF beta-1) was shown to transiently increase the level of expected 900-bp PCR product at 1 h of incubation and decrease the expression at 48 and 72 h with no effect at 24 h. In receptor binding assays using [125I]-IL-1 alpha, TGF beta decreased IL-1R bioactivity at all time points. These results suggest that TGF beta-induced down-regulation of IL-1 RI could be responsible for its ability to antagonize the effect of IL-1 and that TGF beta may have a role in the repair of articular cartilage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect