Dynamic light-scattering measurement of sieving polymer solutions for protein separation on SDS CE
| Autor: | Hideaki Yokoyama, Mitsutoshi Masuda, Keiko Sumitomo, Kohzo Ito, Koichi Mayumi, Yoshinori Yamaguchi, Hiroyuki Minamikawa, Yasuhiro Sakai, Toshimi Shimizu |
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| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Materials science Light Capillary action Clinical Biochemistry Polyacrylamide Analytical chemistry Electrophoresis Capillary Proteins Sodium Dodecyl Sulfate Polymer Biochemistry Analytical Chemistry chemistry.chemical_compound Electrophoresis chemistry Dynamic light scattering Protein purification Homogeneity (physics) Scattering Radiation Hydroxyethyl cellulose |
| Zdroj: | ELECTROPHORESIS. 30:3607-3612 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.200900255 |
| Popis: | We evaluated the mesh size and homogeneity of polymer network by dynamic light scattering and discussed the relationship between the physical properties of polymer network and the protein separation behavior by capillary polymer electrophoresis. We compared three kinds of sieving polymers in solutions with a wide range of molecular weights and concentrations: polyacrylamide and polyethylene oxide as flexible polymers, and hydroxyethyl cellulose as a semiflexible polymer. We found that the mobility of protein was dominated primarily by the mesh size xi, irrespective of the type of sieving polymers, and the peak spacing between protein peaks increased drastically in the range of xi |
| Databáze: | OpenAIRE |
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