Identification and characterization of Aeromonas hydrophila genes encoding the outer membrane receptor of ferrioxamine B and an AraC-type transcriptional regulator
| Autor: | Jun Maki, Hiroshi Tsujibo, Katsushiro Miyamoto, Shigeo Yamamoto, Tatsuya Funahashi, Tomotaka Tanabe |
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| Rok vydání: | 2014 |
| Předmět: |
Transcription
Genetic Iron AraC Transcription Factor Mutant Regulator Deferoxamine Biology Ferric Compounds Applied Microbiology and Biotechnology Biochemistry Substrate Specificity Analytical Chemistry Transcription (biology) Operon Transcriptional regulation Amino Acid Sequence Molecular Biology Gene Sequence Deletion Organic Chemistry Promoter General Medicine Molecular biology Aeromonas hydrophila Complementation Phenotype Multigene Family Energy Metabolism Bacterial outer membrane Bacterial Outer Membrane Proteins Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 78:1777-1787 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2014.932669 |
| Popis: | We found that, under iron-limiting conditions, Aeromonas hydrophila ATCC 7966T could utilize the xenosiderophore desferrioxamine B (DFOB) for growth by inducing the expression of its own outer membrane receptor. Two consecutive genes, desR and desA, were selected as candidates involved in DFOB utilization. The presence of the ferric-uptake regulator boxes in their promoters suggested that these genes are under iron-dependent regulation. Mutation of desA, a gene that encodes the outer membrane receptor of ferrioxamine B, disrupted the growth of the amonabactin-deficient mutant in the presence of DFOB. β-Galactosidase reporter assays and reverse transcriptase-quantitative PCR demonstrated that desR, a gene that encodes an AraC-like regulator homolog is required for the induction of desA transcription in the presence of DFOB and under iron-limiting conditions. The functions of desA and desR were analyzed using complementation experiments. Our data provided evidence that DesA is powered primarily by the TonB2 system. |
| Databáze: | OpenAIRE |
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