Effects of topical erythropoietin on healing experimentally-induced avascular scleral damage in a rabbit model
| Autor: | Sepehr Feizi, Hadi Ebrahimi, Mohammad Ali Javadi, Mozhgan Rezaei Kanavi, Sahar Safari |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Necrosis genetic structures Angiogenesis Antigens Differentiation Myelomonocytic Administration Ophthalmic Apoptosis Neovascularization Cellular and Molecular Neuroscience Antigens CD Ophthalmology Cornea In Situ Nick-End Labeling medicine Animals Fluorescent Antibody Technique Indirect Erythropoietin Wound Healing Retina business.industry Recombinant Proteins eye diseases Sensory Systems Platelet Endothelial Cell Adhesion Molecule-1 Disease Models Animal medicine.anatomical_structure Rabbit model Leukocyte Common Antigens Rabbits sense organs medicine.symptom business Sclera Scleritis medicine.drug |
| Zdroj: | Experimental Eye Research. 190:107898 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2019.107898 |
| Popis: | The present study was designed to investigate the effect of topical erythropoietin on the healing process of induced necrotizing scleritis and to evaluate the ocular side effects of this treatment modality in a rabbit model. Necrotizing scleritis was induced in 8 New Zealand albino rabbits. The animals were then randomly divided into one of two groups: a treated group administered a topical erythropoietin-containing cellulose-based gel every 8 h or a control group treated with a cellulose-based gel without erythropoietin every 8 h. The sizes of the lesions measured at different time points were compared between the groups. After three months, the rabbits’ eyes were enucleated and histologically and immunohistochemically evaluated for angiogenesis and apoptosis. The lesions were completely vascularized in all eyes of the treated group and 50% of eyes of the control group. The mean interval from the induction of scleral necrosis to a complete improvement was 28 days in the treated group and 62.5 days in the control group (P = 0.04). Histological examination revealed that erythropoietin enhanced the improvement of necrotizing scleritis by stimulating angiogenesis and reducing apoptosis. Neovascularization of the cornea, iris, or retina was not observed in the treated group. We observed a significantly faster recovery to complete improvement of necrotizing scleritis in rabbit eyes treated with erythropoietin compared to those of the control group. Treated eyes had a higher rate of complete healing and had no ocular safety concerns. This therapeutic modality represents a promising treatment for scleral necrosis following various types of ocular surgery. |
| Databáze: | OpenAIRE |
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