Matrix metalloproteinase-9 reversibly affects the time course of NMDA-induced currents in cultured rat hippocampal neurons
| Autor: | Leszek Kaczmarek, Piotr Michaluk, Katarzyna Szczuraszek, Paulina Wyrembek, Jerzy W. Mozrzymas, Tomasz Gorkiewicz |
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| Rok vydání: | 2010 |
| Předmět: |
Neurons
biology Chemistry Cognitive Neuroscience Integrin Kinetics Long-Term Potentiation Long-term potentiation Hippocampal formation Hippocampus Receptors N-Methyl-D-Aspartate Rats nervous system Matrix Metalloproteinase 9 biology.protein NMDA receptor Animals Receptor Long-term depression Neuroscience Protein kinase C Cells Cultured |
| Zdroj: | Hippocampus. 20(10) |
| ISSN: | 1098-1063 |
| Popis: | Matrix Metalloproteinase 9 (MMP-9) has been demonstrated to play a crucial role in maintenance of NMDA receptor-dependent LTP and in lateral mobility of these receptors. However, the effect of MMP-9 on NMDA receptor (NMDAR) functional properties is unknown. For this purpose we have investigated the impact of recombinant MMP-9 on the whole-cell NMDAR-mediated current responses in cultured hippocampal neurons. Treatment with MMP-9 induced a reversible acceleration of desensitization and deactivation kinetics but had no effect on current amplitude. Interestingly, phorbol ester, a PKC activator known to enhance NMDAR lateral mobility, induced kinetic changes of currents similar to those produced by MMP-9. In conclusion, our results show that MMP-9 reversibly modulates the NMDAR kinetics and raise a possibility that this modulation could be related to the lateral mobility of these receptors. (C) 2009 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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