Molecular Diversity of Seed-borne Fusarium Species Associated with Maize in India
| Autor: | Siddaiah Chandra Nayaka, Mohammed Aiyaz, Venkataramana Mudili, Vijai Kumar Gupta, Tapani Yli-Mattila, Shetty Thimmappa Divakara, Geromy G. Moore, Siddapura Ramachandrappa Niranjana |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Fusarium mycotoxin contamination Fumonisin gene cluster polymerase-chain-reaction cd-elisa gibberella-moniliformis Article law.invention 03 medical and health sciences chemistry.chemical_compound aspergillus-flavus tef-1 alpha gene law CD-ELISA Genotype Fumonisin Botany Genetics fumonisin production Inter simple sequence repeats Genotyping Genetics (clinical) Polymerase chain reaction southern india Genetic diversity biology UPGMA polyketide synthase gene food and beverages biology.organism_classification Maize risk-factor 030104 developmental biology chemistry sequence alignment tef-1α gene Microsatellite neural-tube defects |
| Zdroj: | Current Genomics |
| ISSN: | 1389-2029 |
| Popis: | A total of 106 maize seed samples were collected from different agro-climatic regions of India. Sixty-two Fusarium isolates were recovered, 90% of which were identified as Fusarium verticillioides based on morphological and molecular characters. Use of the tef-1 alpha gene corrected/refined the morphological species identifications of 11 isolates, and confirmed those of the remaining isolates. Genetic diversity among the Fusarium isolates involved multilocus fingerprinting profiles by Inter Simple Sequence Repeats (ISSR) UP-GMA and tef-1 alpha gene phenetic analyses; for which, we observed no significant differences among the isolates based on geographic origin or fumonisin production; most of the subdivision related to species. Genotyping was performed on the F. verticillioides isolates, using 12 primer sets from the fumonisin pathway, to elucidate the molecular basis of fumonisin production or non-production. One fumonisin-negative isolate, UOMMF-16, was unable to amplify nine of the 12 fumonisin cluster genes tested. We also used the CD-ELISA method to confirm fumonisin production for our 62 Fusarium isolates. Only 15 isolates were found to be fumonisin-negative. Interestingly, genotypic characterization revealed six isolates with various gene deletion patterns that also tested positive for the production of fumonisins via CD-ELISA. Our findings confirm the importance of molecular studies for species delimitation, and for observing genetic and phenotypic diversity, among the Fusaria. |
| Databáze: | OpenAIRE |
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