Serial observations of chronic rotavirus infection in an immunodeficient child
| Autor: | Yoshiichi Minekawa, Kosuke Haruki, Isao Oishi, Kenji Yamazaki, Yoshiyuki Seto, Tsukasa Murakami, Teruo Kimura, Hitokazu Funamoto |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Rotavirus viruses Immunology Reoviridae Biology medicine.disease_cause Microbiology Virus Rotavirus Infections Virology Immunopathology medicine Humans Hepatitis Severe combined immunodeficiency Infant medicine.disease biology.organism_classification Chronic infection Chronic Disease RNA Viral Electrophoresis Polyacrylamide Gel Severe Combined Immunodeficiency Viral disease |
| Zdroj: | Microbiology and immunology. 35(11) |
| ISSN: | 0385-5600 |
| Popis: | Chronic rotavirus infection of an infant with severe combined immunodeficiency (SCID) was studied by virological examinations in association with long-term observation of his symptoms and immune status. During eleven months of hospitalization, the patient was suffering from incurable severe diarrhea with persisting excretion of rotaviruses detected by electron microscopy and the reversed-passive hemagglutination (R-PHA) test and had transient hepatitis symptom despite multiple administrations of human gammaglobulin and high calorie fluids. The detected viruses were morphologically recognized as rotavirus with double capsid structure. Polyacrylamide gel electrophoretic (PAGE) analysis of their genomic RNAs showed the long electropherotype of group A virus with abnormal migration profiles changing considerably from the early to the late phase of illness: (1) The 11th segment became undetectable; (2) the molecular weight of the 6th segment slightly increased; (3) seven to fourteen extra segments appeared; and (4) PAGE patterns of viral genomic RNAs changed every three or four months. These findings suggest that chronic infection with rotavirus accompanied the generation of extra viral genomic segments and their unusual assortments in an immunodeficient host. |
| Databáze: | OpenAIRE |
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