The modulatory effect of Artemisia annua L. on toll-like receptor expression in Acanthamoeba infected mouse lungs
| Autor: | Elżbieta Wandurska-Nowak, Paweł P. Jagodziński, Monika Derda, Edward Hadaś, Agnieszka Kolasa-Wołosiuk, Agnieszka Wojtkowiak-Giera, Danuta Kosik-Bogacka |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DNA Complementary Lung Diseases Parasitic 030231 tropical medicine Immunology Artemisia annua Acanthamoeba Inflammation Biology Real-Time Polymerase Chain Reaction Microbiology Alveolar cells Mice 03 medical and health sciences 0302 clinical medicine Immune system Parenchyma medicine Animals RNA Messenger Lung Mice Inbred BALB C Toll-like receptor Plant Extracts Reverse Transcriptase Polymerase Chain Reaction Toll-Like Receptors Amebiasis Reverse Transcription General Medicine respiratory system 030108 mycology & parasitology biology.organism_classification Immunohistochemistry Toll-Like Receptor 2 Toll-Like Receptor 4 TLR2 Infectious Diseases medicine.anatomical_structure Parasitology medicine.symptom RNA Protozoan |
| Zdroj: | Experimental Parasitology. 199:24-29 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2019.02.011 |
| Popis: | The genus Acanthamoeba, which may cause different infections in humans, occurs widely in the environment. Lung inflammation caused by these parasites induces pulmonary pathological changes such as pulmonary necrosis, peribronchial plasma cell infiltration, moderate desquamation of alveolar cells and partial destruction of bronchial epithelial cells, and presence of numerous trophozoites and cysts among inflammatory cells. The aim of this study was to assess the influence of plant extracts from Artemisia annua L. on expression of the toll-like receptors TLR2 and TLR4 in lungs of mice with acanthamoebiasis. A. annua, which belongs to the family Asteraceae, is an annual plant that grows wild in Asia. In this study, statistically significant changes of expression of TLR2 and TLR4 were demonstrated. In the lungs of infected mice after application of extract from A. annua the expression of TLRs was observed mainly in bronchial epithelial cells, pneumocytes (to a lesser extent during the outbreak of infection), and in the course of high general TLR expression. TLR4 in particular was also visible in stromal cells of lung parenchyma. In conclusion, we confirmed that a plant extract of A. annua has a modulatory effect on components of the immune system such as TLR2 and TLR4. |
| Databáze: | OpenAIRE |
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