Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis
| Autor: | Nobutaka Mitsuda, Masaki Ito, Takamasa Suzuki, Kenichiro Maeo, Nozomi Haga, Kenzo Nakamura, Misato Ohtani, Gerd Jürgens, Minoru Kubo, Taku Demura, Kosuke Kobayashi |
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| Rok vydání: | 2011 |
| Předmět: |
TBX1
G2 Phase Physiology Molecular Sequence Data Arabidopsis Down-Regulation Plant Science Biology Transcription (biology) Gene Expression Regulation Plant Caffeine Genetics MYB Amino Acid Sequence Gene Conserved Sequence Binding Sites Microarray analysis techniques Activator (genetics) Arabidopsis Proteins Qa-SNARE Proteins Development and Hormone Action biology.organism_classification Diploidy Seedlings Mutation Seeds Trans-Activators Cytokinesis Cell Division |
| Zdroj: | Plant physiology. 157(2) |
| ISSN: | 1532-2548 |
| Popis: | R1R2R3-Myb proteins represent an evolutionarily conserved class of Myb family proteins important for cell cycle regulation and differentiation in eukaryotic cells. In plants, this class of Myb proteins are believed to regulate the transcription of G2/M phase-specific genes by binding to common cis-elements, called mitosis-specific activator (MSA) elements. In Arabidopsis (Arabidopsis thaliana), MYB3R1 and MYB3R4 act as transcriptional activators and positively regulate cytokinesis by activating the transcription of KNOLLE, which encodes a cytokinesis-specific syntaxin. Here, we show that the double mutation myb3r1 myb3r4 causes pleiotropic developmental defects, some of which are due to deficiency of KNOLLE whereas other are not, suggesting that multiple target genes are involved. Consistently, microarray analysis of the double mutant revealed altered expression of many genes, among which G2/M-specific genes showed significant overrepresentation of the MSA motif and a strong tendency to be down-regulated by the double mutation. Our results demonstrate, on a genome-wide level, the importance of the MYB3R-MSA pathway for regulating G2/M-specific transcription. In addition, MYB3R1 and MYB3R4 may have diverse roles during plant development by regulating G2/M-specific genes with various functions as well as genes possibly unrelated to the cell cycle. |
| Databáze: | OpenAIRE |
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