Associations between T cell infiltration, T cell receptor clonality, histology and recurrence in renal cell carcinoma
Autor: | Raya Leibowitz, Zohar A. Dotan, Jacob Ramon, Dror Avni, Eduard Fridman, Mordechay Gal, Paula Dobosz, Moran Gadot, Raanan Berger |
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Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine T-Lymphocytes T cell Immunology Receptors Antigen T-Cell Biology 03 medical and health sciences 0302 clinical medicine Renal cell carcinoma Multiplex polymerase chain reaction medicine Humans Immunology and Allergy Receptor Carcinoma Renal Cell Retrospective Studies T-cell receptor Histology Middle Aged medicine.disease Kidney Neoplasms genomic DNA 030104 developmental biology medicine.anatomical_structure Localized disease Cancer research Female ORIGINAL ARTICLES Neoplasm Recurrence Local 030215 immunology |
Zdroj: | Clin Exp Immunol |
ISSN: | 1365-2249 0009-9104 |
Popis: | Summary Renal cell carcinoma (RCC) is comprised of clear-cell (ccRCC) and non-clear-cell (nccRCC) tumors. Despite definitive surgical resection in localized disease, recurrence often occurs. A commercial method based on a multiplex polymerase chain reaction (PCR) assay exclusively targets rearranged T cell receptor (TCR) genes to generate high-throughput sequencing-based data, allowing characterization of the immune repertoire within tumors. In this study we performed a retrospective analysis on archived tumor samples from patients with recurring versus non-recurring T3 ccRCC and on samples from early nccRCC versus ccRCC. Following genomic DNA extraction and multiplex PCR, the fraction of T cells within tumors, the number of unique receptors (‘richness’) and their relative abundances (‘clonality’) were calculated. Statistical significance and correlations were calculated using Student's t-test and Spearman's rho, respectively. Average fraction and clonality of T cells in tumors from non-recurring patients was 2.5- and 4.3-fold higher than in recurring patients (P = 0.025 and P = 0.043, respectively). A significant positive correlation was found between T cell fraction and clonality (Spearman's rho = 0.78, P = 0.008). The average fraction of T cells in ccRCC tumors was 2.8-fold higher than in nccRCC tumors (P = 0.015). Clonality and estimated richness were similar between ccRCC and nccRCC tumors. In summary, recurrence of ccRCC is associated with a lower fraction and clonality of T cells within tumors; nccRCC tumors are more ‘deserted’ than ccRCC, but similar in their ability to generate a clonal T cell repertoire. Our work suggests associations between the characteristics of T cell infiltrate, histology and tumor recurrence. |
Databáze: | OpenAIRE |
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