Functional remineralization of dentin lesions using polymer-induced liquid-precursor process
| Autor: | Anora K, Burwell, Taili, Thula-Mata, Laurie B, Gower, Stefan, Habelitz, Stefan, Habeliz, Michael, Kurylo, Sunita P, Ho, Yung-Ching, Chien, Jing, Cheng, Nancy F, Cheng, Stuart A, Gansky, Sally J, Marshall, Grayson W, Marshall |
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| Přispěvatelé: | Glogauer, Michael |
| Rok vydání: | 2012 |
| Předmět: |
Biomineralization
Anatomy and Physiology Scanning electron microscope Polymers lcsh:Medicine 02 engineering and technology Microscopy Atomic Force Mineralization (biology) Crystallinity Engineering 0302 clinical medicine Dentin lcsh:Science Musculoskeletal System Microstructure Microscopy Multidisciplinary Chemistry Atomic Force 021001 nanoscience & nanotechnology medicine.anatomical_structure Transmission electron microscopy Medicine Materials Characterization medicine.symptom 0210 nano-technology Research Article General Science & Technology Materials Science Material Properties Oral Medicine Biomedical Engineering Mineralogy Bioengineering Biomaterials Natural Materials Lesion 03 medical and health sciences stomatognathic system medicine Humans Bone Biology Remineralisation lcsh:R 030206 dentistry Nanoindentation Dentistry lcsh:Q Physiological Processes Biomedical engineering |
| Zdroj: | PloS one, vol 7, iss 6 Burwell, AK; Thula-Mata, T; Gower, LB; Habeliz, S; Kurylo, M; Ho, SP; et al.(2012). Functional remineralization of dentin lesions using polymer-induced liquid-precursor process. PLoS ONE, 7(6). doi: 10.1371/journal.pone.0038852. UCSF: Retrieved from: http://www.escholarship.org/uc/item/6tb026k8 PLoS ONE, Vol 7, Iss 6, p e38852 (2012) PLoS ONE |
| DOI: | 10.1371/journal.pone.0038852. |
| Popis: | It was hypothesized that applying the polymer-induced liquid-precursor (PILP) system to artificial lesions would result in time-dependent functional remineralization of carious dentin lesions that restores the mechanical properties of demineralized dentin matrix. 140 μm deep artificial caries lesions were remineralized via the PILP process for 7-28 days at 37°C to determine temporal remineralization characteristics. Poly-L-aspartic acid (27 KDa) was used as the polymeric process-directing agent and was added to the remineralization solution at a calcium-to-phosphate ratio of 2.14 (mol/mol). Nanomechanical properties of hydrated artificial lesions had a low reduced elastic modulus (ER= 0.2 GPa) region extending about 70 μm into the lesion, with a sloped region to about 140 μm where values reached normal dentin (18-20 GPa). After 7 days specimens recovered mechanical properties in the sloped region by 51% compared to the artificial lesion. Between 7-14 days, recovery of the outer portion of the lesion continued to a level of about 10 GPa with 74% improvement. 28 days of PILP mineralization resulted in 91% improvement of ERcompared to the artificial lesion. These differences were statistically significant as determined from change-point diagrams. Mineral profiles determined by micro x-ray computed tomography were shallower than those determined by nanoindentation, and showed similar changes over time, but full mineral recovery occurred after 14 days in both the outer and sloped portions of the lesion. Scanning electron microscopy and energy dispersive x-ray analysis showed similar morphologies that were distinct from normal dentin with a clear line of demarcation between the outer and sloped portions of the lesion. Transmission electron microscopy and selected area electron diffraction showed that the starting lesions contained some residual mineral in the outer portions, which exhibited poor crystallinity. During remineralization, intrafibrillar mineral increased and crystallinity improved with intrafibrillar mineral exhibiting the orientation found in normal dentin or bone. © 2012 Burwell et al. |
| Databáze: | OpenAIRE |
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