Correlation Between Morphology, Immunohistochemistry and Molecular Pathology in Hereditary and Sporadic Breast Cancer Cases
Autor: | A Zenios, Andreas Hadjisavvas, Adamos Adamou, Ch. Christodoulou, Kyriacos Kyriacou, Ioanna Zouvani, C. Mikellidou |
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Rok vydání: | 2002 |
Předmět: |
Adult
Pathology medicine.medical_specialty Immunoelectron microscopy Blotting Western Genes BRCA1 Breast Neoplasms Biology Malignancy Pathology and Forensic Medicine Breast cancer Western blot Structural Biology medicine Humans Microscopy Immunoelectron skin and connective tissue diseases Germ-Line Mutation Polymorphism Single-Stranded Conformational Aged medicine.diagnostic_test BRCA1 Protein Molecular pathology Cancer Histology Middle Aged medicine.disease Immunohistochemistry Cyprus Female |
Zdroj: | Ultrastructural Pathology. 26:237-244 |
ISSN: | 1521-0758 0191-3123 |
Popis: | Breast cancer still represents a serious health problem and is currently the most frequent malignancy in the female population in developed countries. In Cyprus, there are 300 new cases annually. In the present study, histology, electron microscopy, immunohistochemistry, and Western blot analysis were used to investigate 100 cases of invasive breast carcinoma. In addition, mutation analysis for the BRCA1 gene was carried out in patient DNA from 26 families with multiple cases of breast/ovarian cancers. Of note are the results of molecular biology which show that there are no germline truncating mutations in the BRCA1 gene in these 26 Cypriot breast cancer families. Furthermore, Western blot analysis revealed the presence of multiple BRCA1 bands in homogenates of tumor and normal tissues, and immunoelectron microscopy showed the presence of nuclear staining for BRCA1 antibodies. |
Databáze: | OpenAIRE |
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