Shigella impairs T lymphocyte dynamics in vivo
| Autor: | Armelle Phalipon, Philippe J. Sansonetti, Ellen T. Arena, Philippe Bousso, Katharina Nothelfer, Gernot Sellge, Wilmara Salgado-Pabón, Elisabetta Frigimelica, Susanna Celli, Pascal Roux |
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| Přispěvatelé: | Pathogénie Microbienne Moléculaire, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Dynamiques des Réponses immunes - Dynamics of Immune Responses, Imagerie Dynamique (Plate-Forme) (PFID), Institut Pasteur [Paris] (IP), Collège de France - Chaire Microbiologie et Maladies infectieuses, Collège de France (CdF (institution)), This work was supported by Institut Pasteur Programme Transversal de Recherche project no. 251, the Domaine d'Intérêt Majeur Malinf-Région Île de France, Agence Nationale de la Recherche project 'PATHIMMUN,' Ministère des Affaires Etrangères Projet P2R, Pasteur Foundation (W.S.-P.), and Philippe Foundation (W.S.-P.). P.J.S. is supported by the European Research Council (ERC) (project Homeopith), he is a Howard Hughes Medical Institute Foreign Scholar., We thank the members of the Pathogénie Microbienne Moléculaire unit for helpful discussions, in particular Claude Parsot and Ilia Belotserkovsky, Patrick M. Schlievert (University of Iowa) for advice and critical reading of the manuscript, Emmanuelle Perret and Anne Danckaert (Platform for Dynamic Imaging Studies) for imaging and data analysis support, Gerald Eberl and Shinichiro Sawa (Institut Pasteur) for reagents and help with fluorescence immunohistochemistry, Sabrina Nabti (Institut National de la Santé et de la Recherche Médicale Unit U932, Institut Curie) for the initial Rag−/− OT-II T-cell receptor transgenic mouse breeding pairs, and Cecile Arrieumerlou and Dirk Bumann (Biozentrum, University Basel) for pMW211 plasmid., Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris], Chaire Microbiologie et Maladies infectieuses |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
CD4-Positive T-Lymphocytes
Priming (immunology) Adaptive Immunity Biology MESH: Mice Knockout Shigella flexneri Type three secretion system Microbiology 03 medical and health sciences 0302 clinical medicine Immune system MESH: Shigella flexneri / physiology Cell Movement MESH: Host-Pathogen Interactions / immunology Animals MESH: Animals MESH: Mice MESH: Cell Movement / immunology Dysentery Bacillary 030304 developmental biology Antigens Bacterial 0303 health sciences Multidisciplinary Effector MESH: Adaptive Immunity MESH: Signal Transduction / immunology imaging MESH: CD4-Positive T-Lymphocytes / immunology T lymphocyte MESH: Dysentery Bacillary / immunology Biological Sciences biochemical phenomena metabolism and nutrition Acquired immune system biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Host-Pathogen Interactions bacteria Female host–pathogen cross-talks Bacterial antigen MESH: Female MESH: Antigens Bacterial / immunology 030215 immunology MESH: Dysentery Bacillary / genetics |
| Zdroj: | Proceedings of the National Academy of Sciences Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 (12), pp.4458-4463. ⟨10.1073/pnas.1300981110⟩ Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2013, 110 (12), pp.4458-4463. ⟨10.1073/pnas.1300981110⟩ |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.1300981110 |
| Popis: | The Gram-negative enteroinvasive bacterium Shigella flexneri is responsible for the endemic form of bacillary dysentery, an acute rectocolitis in humans. S. flexneri uses a type III secretion system to inject effector proteins into host cells, thus diverting cellular functions to its own benefit. Protective immunity to reinfection requires several rounds of infection to be elicited and is short-lasting, suggesting that S. flexneri interferes with the priming of specific immunity. Considering the key role played by T-lymphocyte trafficking in priming of adaptive immunity, we investigated the impact of S. flexneri on T-cell dynamics in vivo. By using two-photon microscopy to visualize bacterium–T-cell cross-talks in the lymph nodes, where the adaptive immunity is initiated, we provide evidence that S. flexneri , via its type III secretion system, impairs the migration pattern of CD4 + T cells independently of cognate recognition of bacterial antigens. We show that bacterial invasion of CD4 + T lymphocytes occurs in vivo, and results in cell migration arrest. In the absence of invasion, CD4 + T-cell migration parameters are also dramatically altered. Signals resulting from S. flexneri interactions with subcapsular sinus macrophages and dendritic cells, and recruitment of polymorphonuclear cells are likely to contribute to this phenomenon. These findings indicate that S. flexneri targets T lymphocytes in vivo and highlight the role of type III effector secretion in modulating host adaptive immune responses. |
| Databáze: | OpenAIRE |
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