Significant improvement of intestinal microbiota of gibel carp (Carassius auratus gibelio) after traditional Chinese medicine feeding

Autor: H. Chen, Francois Joel Gatesoupe, Aihua Li, Xiehao Wang, Dongyue Feng, Qianqian Zhang, Tongtong Li, Yuqing Feng, Zhenbing Wu
Přispěvatelé: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology - Chinese Academy of Sciences, University of Chinese Academy of Sciences, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), College of Biotechnology and Bioengineering, Zhejiang University of Technology, Freshwater Aquaculture Collaborative Innovation Centre of Hubei Province, Huazhong Agricultural University, National Fisheries technical Extension Centre, Ministry of Agriculture, Fisheries Technical Extension Centre of Jiangsu Province, This work was supported by grants from FEBL project (2016FBZ04), Jiangsu Marine and Fisheries Bureau project (D2015-11 and D2017-3), Science and technology basic project of science and technology (2014FY120200) and the Natural Science Foundation of China (no. 30670112 and 31070112)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
intestinal microbiota
Feed additive
030106 microbiology
prévention des maladies
Zoology
médecine traditionnelle
Biology
Gut flora
traditional medicine
Applied Microbiology and Biotechnology
Actinobacteria
03 medical and health sciences
Aquaculture
microbiote
Goldfish
Lactobacillus
Animals
14. Life underwater
Medicine
Chinese Traditional
Carp
gibel carp
food additive
additif alimentaire
Bacteria
business.industry
carp
[SDV.BA]Life Sciences [q-bio]/Animal biology
disease prevention
High-Throughput Nucleotide Sequencing
high-throughput sequencing
Fusobacteria
Biodiversity
General Medicine
biology.organism_classification
6. Clean water
Gastrointestinal Microbiome
3. Good health
carpe
030104 developmental biology
aquaculture
traditional chinese medicine
microbial balance
Proteobacteria
business
Drugs
Chinese Herbal
Biotechnology
Zdroj: Journal of Applied Microbiology
Journal of Applied Microbiology, Wiley, 2018, 124 (3), pp.829-841. ⟨10.1111/jam.13674⟩
ISSN: 1364-5072
1365-2672
DOI: 10.1111/jam.13674⟩
Popis: Aims Increasing attention has been attracted to intestinal microbiota, due to interactions with nutrition, metabolism and immune defence of the host. Traditional Chinese medicine (TCM) feed additives have been applied in aquaculture to improve fish health, but the interaction with fish gut microbiota is still poorly understood. This study aimed to explore the effect of adding TCM in feed on the intestinal microbiota of gibel carp (Carassius auratus gibelio). Methods and results Bacterial communities of 16 fish intestinal contents and one water sample were characterized by high-throughput sequencing and analysis of the V4-V5 region of the 16S rRNA gene. The results showed that the composition and structure of the bacterial community were significantly altered by the TCM feeding. Some phyla increased markedly (Proteobacteria, Actinobacteria, Acidobacteria, etc.), while Fusobacteria were significantly reduced. Concurrently, the richness and diversity of the taxonomic units increased, and the microbiota composition of TCM-treated fish was more homogeneous among individuals. At the genus level, the addition of TCM tended to reduce the incidence of potential pathogens (Aeromonas, Acinetobacter and Shewanella), while stimulating the emergence of some potential probiotics (Lactobacillus, Lactococcus, Bacillus and Pseudomonas). Conclusions These data suggested that the feed additive could regulate the fish intestinal microbiota by reinforcing the microbial balance. Significance and impact of the study This study may provide useful information for further application of TCM for diseases prevention and stress management in aquaculture.
Databáze: OpenAIRE
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