Radiation-Induced Changes of microRNA Expression Profiles in Radiosensitive and Radioresistant Leukemia Cell Lines with Different Levels of Chromosome Abnormalities
| Autor: | Viacheslav Saenko, Wladimir Sibirnyj, Yury Saenko, Eugenia Rastorgueva, Anna Khokhlova, Aleksandr Fomin, Daria Liamina |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
signaling pathway Cancer Research Biology Genome lcsh:RC254-282 Article 03 medical and health sciences 0302 clinical medicine Radioresistance microRNA Gene expression Gene Chromosome localization Chromosome lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Molecular biology radioresistance 030104 developmental biology Oncology radiosensitivity 030220 oncology & carcinogenesis gene expression Signal transduction |
| Zdroj: | Cancers, Vol 9, Iss 10, p 136 (2017) Cancers Cancers; Volume 9; Issue 10; Pages: 136 |
| ISSN: | 2072-6694 |
| Popis: | In our study, we estimate an effect from chromosome aberrations and genome mutations on changes in microRNA expression profiles in cancer cell lines demonstrating different radiosensitivity. Here, cell viability and microRNA spectrum have been estimated 1, 4, and 24 h after irradiation. MiSeq high-throughput sequencing system (Illumina, San Diego, CA, USA) is employed to perform microRNA spectrum estimation. In the K562 cell line, the number of expressed microRNAs in chromosomes demonstrates a more pronounced variation. An analysis of microRNA effects on signaling pathway activity demonstrates differences in post-transcriptional regulation of the expression of genes included into 40 signaling pathways. In the K562 cell line, microRNA dynamics analyzed for their dependence on chromosome localization show a wider scattering of microRNA expression values for a pair of chromosomes compared to the HL-60 cell line. An analysis of microRNAs expression in the K562 and HL-60 cell lines after irradiation has shown that chromosome abnormalities can affect microRNA expression changes. A study of radiation-induced changes of microRNA expression profiles in the K562 and HL-60 cell lines has revealed a dependence of microRNA expression changes on the number of chromosome aberrations and genome mutations. |
| Databáze: | OpenAIRE |
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