Evaluation of the expression of I-FABP and L-FABP in a necrotizing enterocolitis model after the use of Lactobacillus acidophilus
| Autor: | Lourenço Sbragia, Rebeca Lopes Figueira, Ana Leda Bertoncini Simões, Rodrigo Melo Gallindo, Frances Lilian Lanhellas Gonçalves, Lucas Manoel Mangueira Soares |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Pathology Necrosis Blotting Western Fatty Acid-Binding Proteins Gastroenterology Fatty acid-binding protein law.invention Lesion Rats Sprague-Dawley Probiotic Lactobacillus acidophilus Intestinal mucosa law Enterocolitis Necrotizing Internal medicine Medicine Animals Humans business.industry Probiotics Infant Newborn Infant General Medicine medicine.disease Immunohistochemistry digestive system diseases Rats Disease Models Animal Animals Newborn Pediatrics Perinatology and Child Health Necrotizing enterocolitis BIOQUÍMICA lipids (amino acids peptides and proteins) Surgery Female medicine.symptom business Breast feeding Biomarkers |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1531-5037 |
| Popis: | Necrotizing enterocolitis (NEC) is a severe intestinal disease that primarily affects premature babies, leading to high mortality and morbidity. Probiotics represent an important alternative prophylaxis for NEC but its mechanism of action is poorly understood. Moreover, intestinal and liver-type fatty acid-binding proteins (I-FABP and L-FABP) may be utilized because markers of intestinal injury, including NEC. We aimed to evaluate the protection induced by the Lactobacillus acidophilus on the intestines of newborn rats submitted to experimental NEC using I-FABP and L-FABP as biomarkers.Sprague-Dawley newborn rats were divided into three groups: (1) C (control)-breast-fed; (2) NEC-subjected to NEC protocol and (3) NECP-NEC+probiotic. Morphometric, intestinal lesion, immunohistochemistry and Western blotting analysis were performed. Statistical significant differences were considered when p0.05.Intestinal weight was lower in NEC and NECP compared to C (p0.05). Intestinal injury was lower in NECP compared to NEC. Prophylactic probiotic recovered mucosa and muscular layers' thickness to C levels (p0.05). I-FABP and L-FABP expressions in NECP showed intermediate values between C and NEC.L. acidophilus had a protective effect on the development of NEC and FABPs could demonstrate the degree of tissular damage of the intestine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect