Development of a solvent-free analytical method for paracetamol quantitative determination in Blood Brain Barrier in vitro model
| Autor: | Nathalie Buhannic, Antonios Vekris, Elodie Mordelet, Klaus G. Petry, Babette B. Weksler, Marie-Hélène Langlois, Christine Bousses, Pierre-Olivier Couraud, Karen Gaudin, Céline Séguard |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Clinical Biochemistry
Analytical chemistry Blood–brain barrier Sensitivity and Specificity Biochemistry Cell Line Analytical Chemistry In vitro model chemistry.chemical_compound Drug Stability medicine Humans Formate Sample preparation Transcellular Chromatography High Pressure Liquid Acetaminophen Chromatography Reverse-Phase Chromatography Aqueous solution Reproducibility of Results Cell Biology General Medicine Volumetric flow rate medicine.anatomical_structure chemistry Blood-Brain Barrier Paracellular transport |
| Zdroj: | Journal of Chromatography B. 988:20-24 |
| ISSN: | 1570-0232 |
| Popis: | A Reversed Phase-High Performance Liquid Chromatography/Diode Array Detection method was developed and validated for paracetamol quantification in cell culture fluid from an in vitro Blood Brain Barrier model. The chromatographic method and sample preparation were developed using only aqueous solvents. The column was a XTerra RP18 150 × 4.6mm, 3.5 μm with a guard column XTerra RP18 20 × 4.6 mm, 3.5 μm at 35 °C and the mobile phase was composed by 100% formate buffer 20 mM at pH 4 and flow rate was set at 1 mL/min. The detection was at 242 nm. The sample was injected at 10 μL. Validation was performed using the accuracy profile approach. The analytical procedure was validated with the acceptance limits at ± 10% over a range of concentration from 1 to 58 mg L(-1). The procedure was then used in routine to determine paracetamol concentration in a brain blood barrier in vitro model. Application of the Unither paracetamol formulation in Blood Brain Barrier model allowed the determination and comparison of the transcellular passage of paracetamol at 37 °C and 4 °C, that excludes paracellular or non specific leakage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect