Inhibiting prolactin by cabergoline accelerates mammary gland remodeling during the early dry period in dairy cows
| Autor: | A. I. De Prado Taranilla, Vanessa Lollivier, Eva Gandemer, Philippe Lamberton, Marion Boutinaud, Lorraine M. Sordillo, Sandra Wiart, N. Isaka |
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| Přispěvatelé: | Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), CEVA Santé Animale, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University [East Lansing], Michigan State University System-Michigan State University System, Université Européenne de Bretagne (UEB), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
mammary gland Mammary gland cow Lactation dairy cows Animal biology biology Lactoferrin [SDV.BA]Life Sciences [q-bio]/Animal biology drying-off mammary involution prolactin 04 agricultural and veterinary sciences water recession Dairying medicine.anatomical_structure vache laitière Female medicine.drug medicine.medical_specialty Cabergoline Injections Intramuscular Dopamine agonist 03 medical and health sciences Mammary Glands Animal Internal medicine Biologie animale Genetics medicine Animals Involution (medicine) Ergolines Mammary gland involution 0402 animal and dairy science 040201 dairy & animal science Prolactin 030104 developmental biology Endocrinology biology.protein glande mammaire Cattle Animal Science and Zoology tarissement Biomarkers prolactine Food Science |
| Zdroj: | Journal of Dairy Science Journal of Dairy Science, American Dairy Science Association, 2017, 100 (12), pp.9787-9798. ⟨10.3168/jds.2017-12783⟩ Journal of Dairy Science, American Dairy Science Association, 2017, sous presse (sous presse), sous presse. ⟨10.3168/jds.2017-12783⟩ Journal of Dairy Science 12 (100), 9787–9798. (2017) |
| ISSN: | 0022-0302 |
| Popis: | International audience; The inhibition of prolactin release using cabergoline, a dopamine agonist, is an effective strategy to accelerate the changes in mammary secretion composition after drying-off. The objective of this study was to determine how cabergoline may affect mammary tissue remodeling during early involution. Holstein dairy cows were treated with either a single i.m. administration of 5.6 mg of cabergoline (Velactis, Ceva Santé Animale, Libourne, France, n = 7) or placebo (n = 7) at the time of drying-off. Mammary biopsy samples were collected 1 wk before drying-off (d -6), after 30 h of milk accumulation (d 1), and again 8 d following drying-off (d 8) to determine changes in gene expression, lactoferrin content, and cell turnover. Blood and mammary secretion samples were collected at d -6 and again at d 1, 2, 3, 4, 8, and 14 following the abrupt cessation of lactation to evaluate indicators of blood-milk barrier integrity and other markers of mammary tissue remodeling. Cabergoline induced less SLC2A1, BAX, CAPN2, and IGFBP5 mRNA expression. In contrast, cabergoline did not modify changes in cell proliferation and apoptosis. Following the cessation of lactation, changes in mammary secretion composition (Na(+) and K(+)) and blood lactose concentrations were indicative of a loss in the blood-milk barrier function in both treatment groups. Cabergoline treatment affected only Na(+) and K(+) concentrations at d 1, suggesting a moderate increase in tight junction permeability. The increase in the activity of MMP9 and in mammary epithelial cell concentration in mammary secretions was greater in cabergoline-treated cows than in control cows, suggesting more mammary tissue remodeling. The increase in lactoferrin immunostaining in the mammary tissue occurred earlier for cabergoline-treated cows than for control cows, and was essentially localized in the stroma. Changes in some key markers of mammary involution suggest that cabergoline accelerates mammary gland remodeling. Thus, a single injection of cabergoline after the last milking would facilitate drying-off by enhancing mammary gland involution. |
| Databáze: | OpenAIRE |
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