Electrochemical sensor with dry reagents implemented in lab-on-chip for single nucleotide polymorphism detection

Autor:	Tanaka, Hirohuki, Fiorini, Paolo, Jones, Ben, Peeters, Sara, Wiederkehr, Rodrigo Sergio, Majeed, Bivragh, Yaku, Hidenobu, Hiraoka, M, Hiraoka, Maki, Matsuno, Toshinobu, Yamashita, Ichiro
Rok vydání:	2014
Předmět:	Chromatography Physics and Astronomy (miscellaneous) Chemistry General Engineering General Physics and Astronomy Single-nucleotide polymorphism Amplicon Lab-on-a-chip Abo gene Chip Molecular biology Electrochemical gas sensor law.invention Pyrophosphoric acid chemistry.chemical_compound law Reagent
Zdroj:	Japanese Journal of Applied Physics. 53:05FS03
ISSN:	1347-4065 0021-4922
DOI:	10.7567/jjap.53.05fs03
Popis:	We developed an electrochemical (EC) sensor having dry reagents to detect pyrophosphoric acid (PPi) produced as a by-product of a polymerasechain- reaction (PCR) amplicon for single nucleotide polymorphism (SNP) detection. The EC sensor is implementable in a lab-on-chip (LoC) system, and a sensor chip having side-wall electrical connections that enable electrical contacts from the top of the LoC has been developed. We also developed separated on-chip placement of dry reagents divided into three groups in a sensor cavity to suppress background current when there is no PPi. Using this chip, we successfully demonstrated SNP detection in the ABO gene from human blood samples, in combination with the allele-specific PCR amplification method using our developed LoC system ispartof: Japanese Journal of Applied Physics 1, Regular Papers, Short Notes & Review Papers vol:53 issue:5 status: published
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f7c0ac55d962d27e4540c2703cf8e69 https://doi.org/10.7567/jjap.53.05fs03 Zobrazit plný text záznamu