Model simulation and experimental verification of a cation-exchange IgG capture step in batch and continuous chromatography
| Autor: | Massimo Morbidelli, Guido Ströhlein, Pascal Valax, Matthias Jöhnck, Thomas Müller-Späth, Michael Schulte, Henri Kornmann, A. Lamproye, E. Charbaut, Lydia Delegrange, Lars Aumann, Gianni Baer |
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| Rok vydání: | 2011 |
| Předmět: |
Chromatography
Ion exchange Kinetic model Chemistry Elution Organic Chemistry Ion chromatography Antibodies Monoclonal General Medicine Chromatography Ion Exchange Biochemistry Analytical Chemistry Adsorption Models Chemical Mass transfer Immunoglobulin G Batch processing Cation Exchange Resins Saturation (chemistry) |
| Zdroj: | Journal of chromatography. A. 1218(31) |
| ISSN: | 1873-3778 |
| Popis: | The cation-exchange capture step of a monoclonal antibody (mAb) purification process using single column batch and multicolumn continuous chromatography (MCSGP) was modeled with a lumped kinetic model. Model parameters were experimentally determined under analytical and preparative conditions: porosities, retention factors and mass transfer parameters of purified mAb were obtained through a systematic procedure based on retention time measurements. The saturation capacity was determined through peak fitting assuming a Langmuir-type adsorption isotherm. The model was validated using linear batch gradient elutions. In addition, the model was used to simulate the start-up, cyclic steady state and shut down behavior of the continuous capture process (MCSGP) and to predict performance parameters. The obtained results were validated by comparison with suitable experiments using an industrial cell culture supernatant. Although the model was not capable of delivering quantitative information of the product purity, it proved high accuracy in the prediction of product concentrations and yield with an error of less than 6%, making it a very useful tool in process development. |
| Databáze: | OpenAIRE |
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