Restricted myeloperoxidase epitopes drive the adaptive immune response in MPO-ANCA vasculitis
| Autor: | Meghan E. Free, Simon Mallal, William W. Kwok, Kristen Cowens, Benjamin G. Vincent, Mark A. Pilkinton, Olivier M. Lardinois, Eddie A. James, Dante S. Bortone, Susan L. Hogan, Ronald J. Falk, Elizabeth A. McInnis, John Sidney, Bjoern Peters, J. Charles Jennette, Jacob J. Hess, Dominic Ciavatta, Carmen E. Mendoza, Alex J. Guseman, Andrew K. Le, Yichun Hu, Katherine G. Stember, Edita Karosiene |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Vasculitis T cell Immunology Receptors Antigen T-Cell Human leukocyte antigen Biology Adaptive Immunity Autoantigens Epitope Article Antibodies Antineutrophil Cytoplasmic 03 medical and health sciences Epitopes Mice 0302 clinical medicine Immune system medicine Immunology and Allergy Animals Humans Amino Acid Sequence Longitudinal Studies Cells Cultured Autoantibodies Peroxidase 030203 arthritis & rheumatology T-cell receptor Autoantibody Acquired immune system 030104 developmental biology medicine.anatomical_structure biology.protein Leukocytes Mononuclear Antibody |
| Zdroj: | J Autoimmun |
| ISSN: | 1095-9157 |
| Popis: | Background Treatment of autoimmune diseases has relied on broad immunosuppression. Knowledge of specific interactions between human leukocyte antigen (HLA), the autoantigen, and effector immune cells, provides the foundation for antigen-specific therapies. These studies investigated the role of HLA, specific myeloperoxidase (MPO) epitopes, CD4+ T cells, and ANCA specificity in shaping the immune response in patients with anti-neutrophil cytoplasmic autoantibody (ANCA) vasculitis. Methods HLA sequence-based typing identified enriched alleles in our patient population (HLA-DPB1*04:01 and HLA-DRB4*01:01), while in silico and in vitro binding studies confirmed binding between HLA and specific MPO epitopes. Class II tetramers with MPO peptides were utilized to detect autoreactive CD4+ T cells. TCR sequencing was performed to determine the clonality of T cell populations. Longitudinal peptide ELISAs assessed the temporal nature of anti-MPO447-461 antibodies. Solvent accessibility combined with chemical modification determined the buried regions of MPO. Results We identified a restricted region of MPO that was recognized by both CD4+ T cells and ANCA. The autoreactive T cell population contained CD4+CD25intermediateCD45RO+ memory T cells and secreted IL-17A. T cell receptor (TCR) sequencing demonstrated that autoreactive CD4+ T cells had significantly less TCR diversity when compared to naive and memory T cells, indicating clonal expansion. The anti-MPO447-461 autoantibody response was detectable at onset of disease in some patients and correlated with disease activity in others. This region of MPO that is targeted by both T cells and antibodies is not accessible to solvent or chemical modification, indicating these epitopes are buried. Conclusions These observations reveal interactions between restricted MPO epitopes and the adaptive immune system within ANCA vasculitis that may inform new antigen-specific therapies in autoimmune disease while providing insight into immunopathogenesis. |
| Databáze: | OpenAIRE |
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