Significance of cytokines and CD68-positive microparticles in immune thrombocytopenic purpura
| Autor: | Mutsumasa Yanabu, Tetsuya Miyake, Yasuhiko Miyazaki, Kaoruko Katsura, Hideo Kagawa, Shirou Fukuhara, Hiroshi Ichiyoshi, Xie Gui Lan, Hirofumi Kido, Shosaku Nomura |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Adolescent Lipopolysaccharide medicine.medical_treatment Antigens Differentiation Myelomonocytic Biology chemistry.chemical_compound Immune system Antigen Antigens CD medicine Humans Platelet Aged Cell Line Transformed U937 cell Monocyte Hematology General Medicine Middle Aged Flow Cytometry medicine.disease Thrombocytopenic purpura Cytokine medicine.anatomical_structure Purpura Thrombocytopenic chemistry Immunology Cytokines Female |
| Zdroj: | European Journal of Haematology. 55:49-56 |
| ISSN: | 1600-0609 0902-4441 |
| Popis: | We investigated the significance of cytokines (soluble interleukin-2 receptor, granulocyte-macrophage colony-stimulating factor, interleukin-6, and interferon-gamma) and CD68-positive microparticles in immune thrombocytopenic purpura. Cytokines were measured by enzyme-linked immunosorbent assay and microparticles were detected by flow cytometry. CD68 expression by histiocytic U937 cells incubated with lipopolysaccharide or cytokines was also assessed in a control study. The level of CD68-positive microparticles was significantly higher in the patients with thrombocytopenia than in normal controls (p0.01). The soluble interleukin-2 receptor level was also significantly higher in patients than in controls (p0.01), but the other cytokines did not show a significant difference. However, patients with severe thrombocytopenia (platelet count20,000/microliters) had significantly higher levels of granulocyte-macrophage colony-stimulating factor and interleukin-6 than the controls (p0.05). When opsonized platelets were incubated with activated U937 cells, lipopolysaccharide and granulocyte-macrophage colony-stimulating factor caused an increase of CD68-positive microparticles in the supernatant. These results suggest that granulocyte-macrophage colony-stimulating factor is released by activated T cells in immune thrombocytopenic purpura and activates monocyte/macrophage phagocytosis, resulting in an increase of circulating CD68-positive microparticles and enhanced platelet destruction. |
| Databáze: | OpenAIRE |
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