Direct and Fast Assessment of Antimicrobial Surface Activity Using Molecular Dynamics Simulation and Time-Lapse Imaging
| Autor: | Ramon Reigada, Rafaël Sibilo, Carlo Manzo, Ilaria Mannelli, Mehmet Alican Noyan, Prantik Mazumder, Valerio Pruneri |
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| Rok vydání: | 2020 |
| Předmět: |
Chemistry
Scanning electron microscope Surface Properties 010401 analytical chemistry Microbial Sensitivity Tests Molecular Dynamics Simulation 010402 general chemistry 01 natural sciences Time-Lapse Imaging 0104 chemical sciences Analytical Chemistry Anti-Bacterial Agents Molecular dynamics Microscopy Fluorescence Biophysics Escherichia coli Antimicrobial surface |
| Zdroj: | Analytical chemistry. 92(10) |
| ISSN: | 1520-6882 |
| Popis: | With the alarming rise of antimicrobial resistance, studies on bacteria-surface interactions are both relevant and timely. Scanning electron microscopy and colony forming unit counting are commonly used techniques but require sophisticated sample preparation and long incubation time. Here, we present a direct method based on molecular dynamics simulation of nanostructured surfaces providing in silico predictions, complemented with time-lapse fluorescence imaging to study live interactions of bacteria at the membrane-substrate level. We evaluate its effectiveness in predicting and statistically analyzing the temporal evolution and spatial distribution of prototypical bacteria with costained nucleoids and membranes ( |
| Databáze: | OpenAIRE |
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