Effect of bone morphogenetic proteins 2 and 4 on survival and development of bovine secondary follicles cultured in vitro
| Autor: | A. W. B. Silva, Valdevane Rocha Araújo, J. R. V. Silva, G.L. Vasconcelos, E. V. da Cunha, G.B. Sousa, L.R.F. Melo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
animal structures Cell Survival Bone Morphogenetic Protein 2 Ovary Bone Morphogenetic Protein 4 Bone morphogenetic protein Bone morphogenetic protein 2 Andrology 03 medical and health sciences Follicle Oogenesis Ovarian Follicle Food Animals medicine Animals Small Animals Cyclin B1 Cells Cultured Equine Chemistry fungi Bone Morphogenetic Protein Receptors biological factors BMPR1A In vitro BMPR1B 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation embryonic structures Oocytes Cattle Female Animal Science and Zoology |
| Zdroj: | Theriogenology. 110:44-51 |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2017.12.032 |
| Popis: | This study evaluated the effect of bone morphogenetic proteins 2 (BMP2) and 4 (BMP2) on follicle development and mRNA expression for GDF9, Cyclin B1, BMPR1A, BMPR1B, BMPRII, FSHR and SMAD1 in bovine secondary follicles cultured in vitro. Isolated secondary follicles were cultured for 18 days in TCM199+ medium alone or supplemented with BMP2 (10 ng/mL), BMP4 (100 ng/mL) or combination of both BMP2 and 4. Real-time PCR was used to analyze mRNA levels in fresh and cultured follicles. After 18 days of culture, follicles cultured with BMP2 alone or with BMP4 alone had larger diameters when compared to control (P |
| Databáze: | OpenAIRE |
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