Photodynamic and Antibiotic Therapy in Combination to Fight Biofilms and Resistant Surface Bacterial Infections

Autor: Giovanna Maria Pierantoni, Giuseppe Palumbo, Maria Rosaria Catania, Emanuela Roscetto, Ilaria Postiglione, Adriana Vollaro, Amata A. Soriano, Federica Barra
Přispěvatelé: Barra, Federica, Roscetto, Emanuela, Soriano, AMATA AMY, Vollaro, Adriana, Postiglione, Ilaria, Pierantoni, GIOVANNA MARIA, Palumbo, Giuseppe, Catania, MARIA ROSARIA
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Light
medicine.drug_class
medicine.medical_treatment
Gram-positive bacteria
Antibiotics
Photodynamic therapy
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Catalysis
Article
Microbiology
combination therapy
lcsh:Chemistry
Inorganic Chemistry
chemistry.chemical_compound
Staphylococcus epidermidis
Drug Resistance
Bacterial
medicine
Photosensitizer
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Gentamicin
Spectroscopy
Microscopy
Confocal
Photosensitizing Agents
Protoporphyrin IX
Bacteria
Organic Chemistry
General Medicine
biology.organism_classification
3. Good health
Computer Science Applications
Anti-Bacterial Agents
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Photochemotherapy
photodynamic therapy
Staphylococcus aureus
5-aminolevulinic acid
Biofilms
Gentamicins
medicine.drug
Zdroj: International journal of molecular sciences
16 (2015): 20417–20430.
info:cnr-pdr/source/autori:Barra, Federica; Roscetto, Emanuela; Soriano, Amata A.; Vollaro, Adriana; Postiglione, Ilaria; Pierantoni, Giovanna Maria; Palumbo, Giuseppe; Catania, Maria Rosaria/titolo:Photodynamic and Antibiotic Therapy in Combination to Fight Biofilms and Resistant Surface Bacterial Infections/doi:/rivista:International journal of molecular sciences (Print)/anno:2015/pagina_da:20417/pagina_a:20430/intervallo_pagine:20417–20430/volume:16
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 16, Iss 9, Pp 20417-20430 (2015)
Volume 16
Issue 9
Pages 20417-20430
Popis: Although photodynamic therapy (PDT), a therapeutic approach that involves a photosensitizer, light and O2, has been principally considered for the treatment of specific types of cancers, other applications exist, including the treatment of infections. Unfortunately, PDT does not always guarantee full success since it exerts lethal effects only in cells that have taken up a sufficient amount of photosensitizer and have been exposed to adequate light doses, conditions that are not always achieved. Based on our previous experience on the combination PDT/chemotherapy, we have explored the possibility of fighting bacteria that commonly crowd infected surfaces by combining PDT with an antibiotic, which normally does not harm the strain at low concentrations. To this purpose, we employed 5-aminolevulinic acid (5-ALA), a pro-drug that, once absorbed by proliferating bacteria, is converted into the natural photosensitizer Protoporphyrin IX (PpIX), followed by Gentamicin. Photoactivation generates reactive oxygen species (ROS) which damage or kill the cell, while Gentamicin, even at low doses, ends the work. Our experiments, in combination, have been highly successful against biofilms produced by several Gram positive bacteria (i.e., Staphylococcus aureus, Staphylococcus epidermidis, etc.). This original approach points to potentially new and wide applications in the therapy of infections of superficial wounds and sores.
Databáze: OpenAIRE
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