Porous graphitic carbon chromatography/tandem mass spectrometric determination of cytarabine in mouse plasma
| Autor: | Christine J. G. Duncan, Yunsheng Hsieh, Jean-Marc Brisson |
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| Rok vydání: | 2007 |
| Předmět: |
Analyte
Antimetabolites Antineoplastic Spectrometry Mass Electrospray Ionization Chromatography Tandem Chemistry Electrospray ionization Organic Chemistry Analytical chemistry Cytarabine Drug Evaluation Preclinical Plasma Mass spectrometry Tandem mass spectrometry High-performance liquid chromatography Analytical Chemistry Mice Tandem Mass Spectrometry Ionization Animals Graphite Spectroscopy Chromatography High Pressure Liquid Injections Intraperitoneal |
| Zdroj: | Rapid communications in mass spectrometry : RCM. 21(5) |
| ISSN: | 0951-4198 |
| Popis: | A high-performance liquid chromatography (HPLC) system using a porous graphitic carbon (PGC) stationary phase interfaced with an electrospray ionization (ESI) source and a tandem mass spectrometer (MS/MS) for the analysis of cytarabine (ara-C) in mouse plasma samples has been developed in support of a pharmacodynamic study. The graphitized carbon column was adopted for the separation of ara-C and endogenous peaks from mouse plasma samples under the reversed-phase phase mode in liquid chromatography. The retention characteristics of the PGC column and the ionization efficiencies of all analytes based on the experimental factors such as the composition of mobile phases were investigated. The potential of ionization suppression resulting from the endogenous biological matrices on the PGC column during HPLC/ESI-MS/MS was investigated using post-column infusion. The concentrations of ara-C in mouse plasma obtained by using PGC-HPLC/MS/MS and ion-pairing HPLC/MS/MS were found to be in good agreement in terms of analytical accuracy. |
| Databáze: | OpenAIRE |
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