Influenza Antigens NP and M2 Confer Cross Protection to BALB/c Mice against Lethal Challenge with H1N1, Pandemic H1N1 or H5N1 Influenza A Viruses
Autor: | Thomas E Hickey, Sonja Leyrer, Hanné Andersen, Nathan D. Grubaugh, Nutan Mytle, Jon R Inglefield, Andrea M. Harris, Michael J. Lacy, Hang Lu, Tina Guina, Jacob T. Minang, Mario H. Skiadopoulos, Zhidong Ma |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
viruses
Cross Protection Genetic Vectors Hemagglutinin (influenza) medicine.disease_cause Antibodies Viral Microbiology Virus Antigenic drift Article Viroporin Proteins Viral Matrix Proteins chemistry.chemical_compound Influenza A Virus H1N1 Subtype Antigen Orthomyxoviridae Infections Virology vaccine matrix protein 1 medicine Influenza A virus matrix protein 2 Animals modified Vaccinia virus Ankara (MVA) hemagglutinin influenza A Antigens Viral Pandemics nucleoprotein Mice Inbred BALB C biology Influenza A Virus H5N1 Subtype Vaccination Nucleocapsid Proteins Influenza A virus subtype H5N1 QR1-502 Nucleoprotein Infectious Diseases chemistry Influenza Vaccines biology.protein Female Vaccinia conserved antigens |
Zdroj: | Viruses Volume 13 Issue 9 Viruses, Vol 13, Iss 1708, p 1708 (2021) |
ISSN: | 1999-4915 |
Popis: | Influenza hemagglutinin (HA) is considered a major protective antigen of seasonal influenza vaccine but antigenic drift of HA necessitates annual immunizations using new circulating HA versions. Low variation found within conserved non-HA influenza virus (INFV) antigens may maintain protection with less frequent immunizations. Conserved antigens of influenza A virus (INFV A) that can generate cross protection against multiple INFV strains were evaluated in BALB/c mice using modified Vaccinia virus Ankara (MVA)-vectored vaccines that expressed INFV A antigens hemagglutinin (HA), matrix protein 1 (M1), nucleoprotein (NP), matrix protein 2 (M2), repeats of the external portion of M2 (M2e) or as tandem repeats (METR), and M2e with transmembrane region and cytoplasmic loop (M2eTML). Protection by combinations of non-HA antigens was equivalent to that of subtype-matched HA. Combinations of NP and forms of M2e generated serum antibody responses and protected mice against lethal INFV A challenge using PR8, pandemic H1N1 A/Mexico/4108/2009 (pH1N1) or H5N1 A/Vietnam/1203/2004 (H5N1) viruses, as demonstrated by reduced lung viral burden and protection against weight loss. The highest levels of protection were obtained with NP and M2e antigens delivered as MVA inserts, resulting in broadly protective immunity in mice and enhancement of previous natural immunity to INFV A. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |