FadA-positive Fusobacterium nucleatum is prevalent in biopsy specimens of Iranian patients with colorectal cancer
| Autor: | Mojgan Forootan, Negin Kashani, A.T. Bezmin Abadi, Farid Rahimi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Colorectal cancer 030106 microbiology colorectal cancer Microbiology Gastroenterology Inflammatory bowel disease lcsh:Infectious and parasitic diseases Lesion 03 medical and health sciences stomatognathic system Internal medicine fusobacterium nucleatum Biopsy Medicine lcsh:RC109-216 fadA medicine.diagnostic_test biology business.industry medicine.disease biology.organism_classification digestive system diseases stomatognathic diseases PCR 030104 developmental biology Infectious Diseases iranian patients Original Article medicine.symptom Fusobacterium nucleatum business |
| Zdroj: | New Microbes and New Infections, Vol 34, Iss, Pp-(2020) New Microbes and New Infections |
| ISSN: | 2052-2975 |
| Popis: | Fusobacterium nucleatum has been increasingly implicated as a causative agent of various diseases, such as inflammatory bowel disease. Moreover, the gastrointestinal tracts of patients with colorectal cancer (CRC) also have been shown to be colonized by this bacterium. We aimed to determine the prevalence of F. nucleatum among CRC and non-CRC Iranian patients and to investigate potential associations between fadA-positive F. nucleatum and diagnosed CRC cases. Eighty patients admitted to two main hospitals in Tehran, Iran, were enrolled. The patients were aged between 20 and 75 and were diagnosed by a gastroenterologist. A trained surgeon used standard surgical protocols to collect two CRC biopsy samples per patient. One of the samples was used for pathologic examination, and the other was subjected to DNA extraction and PCR. Lesion colonization by F. nucleatum and expression of its major virulence factor, fadA, were investigated. The fadA-positive F. nucleatum strain was absent in all the lesions obtained from non-CRC patients. All patients with lesions that were colonized with fadA-positive F. nucleatum were diagnosed as CRC (p |
| Databáze: | OpenAIRE |
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