Sulforaphane modulates TGFβ2-induced conjunctival fibroblasts activation and fibrosis by inhibiting PI3K/Akt signaling
| Autor: | Zi-Yao Xia, Han-Ruo Liu, Ning-Li Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
transforming growth factor β2
biology business.industry Cell growth Akt/PKB signaling pathway sulforaphane pi3k/akt signaling Molecular biology Fibronectin Ophthalmology chemistry.chemical_compound Basic Research medicine.anatomical_structure chemistry lcsh:Ophthalmology lcsh:RE1-994 biology.protein Medicine Viability assay human conjunctival fibroblasts business Fibroblast Protein kinase B PI3K/AKT/mTOR pathway Sulforaphane |
| Zdroj: | International Journal of Ophthalmology, Vol 13, Iss 10, Pp 1505-1511 (2020) Int J Ophthalmol |
| ISSN: | 2227-4898 2222-3959 |
| Popis: | AIM: To examine the effects of sulforaphane on fibrotic changes of transforming growth factor (TGFβ2) induced human conjunctival fibroblast (HConFs). METHODS: HConFs were cultured and divided into control, TGFβ2 (1 ng/mL), sulforaphane and TGFβ2+sulforaphane groups. Cell viability and apoptosis were detected using the MTT and ApoTox-Glo Triplex assay. Cell migration was detected using scratch and Transwell assay. Real-time quantitative PCR method was used to evaluate mRNA expression of TGFβ2, matrix metalloproteinase-2 (MMP2), myosin light chain kinase (MYLK), integrin αV, integrin α5, fibronectin 1 and α-smooth muscle actin (α-SMA). The protein expression of α-SMA, p-PI3K, PI3K, p-Akt, and Akt were detected by Western blot. RESULTS: The proliferation of HConFs was significantly (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|