Noninvasive Molecular Imaging of Cell Death in Myocardial Infarction using In-111-GSAO
| Autor: | Jagat Narula, Jun Zhou, Navneet Narula, Tsutomu Imaizumi, Riemer H. J. A. Slart, Raghu Pandurangi, Nobuhiro Tahara, Chris P. M. Reutelingsperger, Tiziano M. Scarabelli, Hans J. de Haas, Annapoorna Kini, Mary Dyszlewski, Takayoshi Yamaki, H. Reinier Zandbergen, Valentin Fuster, Artiom Petrov |
|---|---|
| Přispěvatelé: | Banyu Life Science Foundation International, Dutch Heart Association, National Institutes of Health (Estados Unidos), Vascular Ageing Programme (VAP), Cardiovascular Centre (CVC), Translational Immunology Groningen (TRIGR), Carim, RS: CARIM - R1 - Thrombosis and haemostasis, RS: CARIM - R2 - Cardiac function and failure, CTC, Biochemie, Cardiologie |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Programmed cell death Pathology medicine.medical_specialty Necrosis education Myocardial Infarction Indium Arsenicals Article MECHANISMS INCREASED CARDIOMYOCYTE APOPTOSIS Mice In vivo MAGNETIC-RESONANCE Medicine Animals Annexin A5 REPERFUSION INJURY Radionuclide Imaging IN-VIVO SHOCK PROTEIN-90 ALKYLATOR Multidisciplinary business.industry Colocalization Technetium medicine.disease DILATED CARDIOMYOPATHY Glutathione 3. Good health Cell biology Molecular Imaging Disease Models Animal ISCHEMIC-MYOCARDIUM Microscopy Fluorescence ANNEXIN A5 Apoptosis HEART-FAILURE Rabbits medicine.symptom business Reperfusion injury Intracellular |
| Zdroj: | Scientific Reports Scientific Reports, 4:6826. Nature Publishing Group Repisalud Instituto de Salud Carlos III (ISCIII) |
| ISSN: | 2045-2322 |
| Popis: | Acute insult to the myocardium is associated with substantial loss of cardiomyocytes during the process of myocardial infarction. In this setting, apoptosis (programmed cell death) and necrosis may operate on a continuum. Because the latter is characterized by the loss of sarcolemmal integrity, we propose that an appropriately labeled tracer directed at a ubiquitously present intracellular moiety would allow non-invasive definition of cardiomyocyte necrosis. A trivalent arsenic peptide, GSAO (4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid), is capable of binding to intracellular dithiol molecules such as HSP90 and filamin-A. Since GSAO is membrane impermeable and dithiol molecules abundantly present intracellularly, we propose that myocardial localization would represent sarcolemmal disruption or necrotic cell death. In rabbit and mouse models of myocardial infarction and post-infarct heart failure, we employed In-111-labelled GSAO for noninvasive radionuclide molecular imaging. In-111-GSAO uptake was observed within the regions of apoptosis seeking agent-(99)mTc-Annexin A5 uptake, suggesting the colocalization of apoptotic and necrotic cell death processes. This research was supported by a research grant from the International Research Fund for Subsidy of Kyusyu University School of Medicine Alumni and the Banyu Fellowship Program sponsored by Banyu Life Science Foundation International to N. Tahara, and research grants from the foundation ``De Drie Lichten´´ and the Dutch Heart Association (Dr. E. Dekker student Grant) to H. de Haas. DTPA-GSAO, DTPA-GSCA, Cy5.5 GSAO were supplied by Covidien Imaging Solutions. We thank H.H. Boersma for performing blood clearance excellent statistical support we thank E. Bagiella, supported by National Institutes of Health Grant UL1TR000067. Sí |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|