Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein
| Autor: | Michael Riggs, K. Ferguson, Jeffrey E. Gerst, Jeffrey Field, R. Ballester, Scott Powers, Graeme Bolger, Anne Vojtek, T. Michaeli, Tohru Kataoka, B. Wheland, Michael Wigler, Linda Rodgers, Ilse Wieland, John J. Colicelli |
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| Rok vydání: | 1990 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Gs alpha subunit Genotype Protein subunit Blotting Western Genes Fungal Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae Biology General Biochemistry Genetics and Molecular Biology ADCY10 Fungal Proteins Adenylyl cyclase chemistry.chemical_compound Complementary DNA Amino Acid Sequence Cloning Molecular DNA Fungal Cyclase-associated protein family Base Sequence ADCY9 Molecular biology Enzyme Activation Molecular Weight Phenotype chemistry Biochemistry ras Proteins cAMP-dependent pathway Oligonucleotide Probes Adenylyl Cyclases Plasmids |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(90)90812-s |
| Popis: | Adenylyl cyclase from S. cerevisiae contains at least two subunits, a 200 kd catalytic subunit and a subunit with an apparent molecular size of 70 kd, which we now call CAP (cyclase-associated protein). We cloned a cDNA encoding CAP by screening a yeast cDNA expression library in E. coli with antisera raised against the purified protein. The cDNA contained an open reading frame capable of encoding a 526 amino acid protein that is not homologous to any sequences in the current data bases. Adenylyl cyclase activity in membranes from cells that lacked CAP was not stimulated by RAS2 proteins in vitro. These results suggest that CAP is required for at least some aspects of the RAS-responsive signaling system. Mutants lacking CAP had four additional phenotypes that appear to be unrelated to effects of the RAS/adenylyl cyclase pathway: the inability to grow on rich medium (YPD), temperature sensitivity on minimal medium, sensitivity to nitrogen starvation, and a swollen cell morphology. |
| Databáze: | OpenAIRE |
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