Incorporation of SPION‐casein core‐shells into silk‐fibroin nanofibers for cardiac tissue engineering
Autor: | Mousa Kehtari, Iman Rad, Masoud Soleimani, Asieh Heirani-Tabasi, Milad Salimi Bani, Hojjatollah Nazari, Maryam Hajiabbas, Seyed Hossein Ahmadi Tafti, Mahnaz Nazari, Vahid Pirhajati Mahabadi |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Surface Properties Scanning electron microscope Nanofibers Fibroin Biochemistry Nanocomposites Contact angle Mice 03 medical and health sciences 0302 clinical medicine Microscopy Electron Transmission X-Ray Diffraction Tissue engineering Tensile Strength Animals Molecular Biology Cell Nucleus Tissue Engineering Tissue Scaffolds Chemistry Myocardium Cell Differentiation Heart Cell Biology Electrospinning 030104 developmental biology Transmission electron microscopy 030220 oncology & carcinogenesis Nanofiber Microscopy Electron Scanning Biophysics Magnetic nanoparticles Magnetic Iron Oxide Nanoparticles Stress Mechanical Fibroins |
Zdroj: | Journal of Cellular Biochemistry. 121:2981-2993 |
ISSN: | 1097-4644 0730-2312 |
DOI: | 10.1002/jcb.29553 |
Popis: | Mimicking the structure of extracellular matrix (ECM) of myocardium is necessary for fabrication of functional cardiac tissue. The superparamagnetic iron oxide nanoparticles (SPIONs, Fe3 O4 ), as new generation of magnetic nanoparticles (NPs), are highly intended in biomedical studies. Here, SPION NPs (1 wt%) were synthesized and incorporated into silk-fibroin (SF) electrospun nanofibers to enhance mechanical properties and topography of the scaffolds. Then, the mouse embryonic cardiac cells (ECCs) were seeded on the scaffolds for in vitro studies. The SPION NPs were studied by scanning electron microscope (SEM), X-ray diffraction (XRD), and transmission electron microscope (TEM). SF nanofibers were characterized after incorporation of SPIONs by SEM, TEM, water contact angle measurement, and tensile test. Furthermore, cytocompatibility of scaffolds was confirmed by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. SEM images showed that ECCs attached to the scaffolds with elongated morphologies. Also, the real-time PCR and immunostaining studies approved upregulation of cardiac functional genes in ECCs seeded on the SF/SPION-casein scaffolds including GATA-4, cardiac troponin T, Nkx 2.5, and alpha-myosin heavy chain, compared with the ones in SF. In conclusion, incorporation of core-shells in SF supports cardiac differentiation, while has no negative impact on ECCs' proliferation and self-renewal capacity. |
Databáze: | OpenAIRE |
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