Albumin nanoparticles for glutathione-responsive release of cisplatin: New opportunities for medulloblastoma
| Autor: | Francesca Iemma, Manuela Curcio, Umile Gianfranco Spizzirri, Giuseppe Cirillo, Alessandra Vacca, Elisabetta Ferretti, Zein Mersini Besharat, Giuseppina Catanzaro, Luana Abballe, Nevio Picci |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Keratinocytes
Cell Survival Pharmaceutical Science Antineoplastic Agents 02 engineering and technology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cystamine Cell Line Tumor medicine Humans Viability assay Bovine serum albumin Cerebellar Neoplasms Albumin Nanoparticles Cisplatin Drug Carriers biology Chemistry Serum Albumin Bovine Glutathione 021001 nanoscience & nanotechnology Drug Liberation HaCaT Albumin Nanoparticles Redox responsivity Glutathione Biocompatibility Medulloblastoma Cisplatin Redox responsivity Biochemistry 030220 oncology & carcinogenesis Drug delivery Cancer cell Biophysics biology.protein Nanoparticles Biocompatibility 0210 nano-technology Oxidation-Reduction Medulloblastoma medicine.drug |
| Zdroj: | International Journal of Pharmaceutics. 517:168-174 |
| ISSN: | 0378-5173 |
| Popis: | Redox-responsive nanoparticles were synthesized by desolvation of bovine serum albumin followed by disulfide-bond crosslinking with N , N ʹ-Bis (acryloyl) cystamine. Dynamic light scattering and transmission electron microscopy studies revealed spherical nanoparticles (mean diameter: 83 nm, polydispersity index: 0.3) that were glutathione-responsive. Confocal microscopy revealed rapid, efficient internalization of the nanoparticles by Daoy medulloblastoma cells and healthy controls (HaCaT keratinocytes). Cisplatin-loaded nanoparticles with drug:carrier ratios of 5%, 10%, and 20% were tested in both cell lines. The formulation with the highest drug:carrier ratio reduced Daoy and HaCaT cell viability with IC 50 values of 6.19 and 11.17 μg mL −1 , respectively. The differential cytotoxicity reflects the cancer cells’ higher glutathione content, which triggers more extensive disruption of the disulfide bond-mediated intra-particle cross-links, decreasing particle stability and increasing their cisplatin release. These findings support continuing efforts to improve the safety and efficacy of antineoplastic drug therapy for pediatric brain tumors using selective nanoparticle-based drug delivery systems. |
| Databáze: | OpenAIRE |
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