Semen Creatine and Creatine Kinase Activity as an Indicator of Sperm Quality
Autor: | Moncef Feki, Souheil Omar, Mohamed Bassem Hammami, Hamadi Ben Aribia, Fahmi Nasrallah, Haifa Sanhaji |
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Rok vydání: | 2020 |
Předmět: |
Male
Infertility endocrine system Motility Semen Creatine Asthenozoospermia General Biochemistry Genetics and Molecular Biology Andrology chemistry.chemical_compound Humans Medicine Child Creatine Kinase Infertility Male Sperm motility biology urogenital system business.industry medicine.disease Spermatozoa Sperm chemistry Sperm Motility biology.protein Creatine kinase business |
Zdroj: | Clinical Laboratory. 66 |
ISSN: | 1433-6510 |
Popis: | Background The role of creatine (Cr) and creatine kinase (CK) in sperm function remains unclear. The study aimed to assess Cr and CK in seminal plasma and test their association with sperm characteristics. Methods The study included 62 males with couple's infertility and 26 males who have already fathered children. Semen Cr and CK were assessed by GC-MS and spectrophotometry, respectively. Seminogram parameters were analyzed using conventional methods. Results Cytomorphologic analysis of sperm showed normozoospermia in 53 men (NS) and an asthenozoospermia (AS) in 35 men. Semen Cr was high with no significant difference between the two groups (791 ± 342 and 744 ± 422 µmol/L, respectively). However, semen CK activity was higher in AS group (1,360 ± 1,050 vs. 830 ± 580 U/L, p = 0.013). Semen Cr was positively related to progressive motility (r = 0.284; p = 0.010). Semen CK was negatively correlated with sperm concentration (r = -0.29; p = 0.01), progressive motility (r = -0.26; p = 0.03), and the percentage of abnormal spermatozoa (r = -0.28; p = 0.02). Conclusions Semen contains high amounts of Cr and increased CK activity. Low semen Cr is associated with reduced sperm motility while high CK activity is associated with poor sperm quality. The findings suggest that Cr is of importance for sperm metabolism and that Cr supplementation could be useful in males with poor quality sperm. |
Databáze: | OpenAIRE |
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