Expression of CCL2 and CCR2 in the hippocampus and the interventional roles of propofol in rat cerebral ischemia/reperfusion
| Autor: | Jian‑Min Jing, Wei‑Wei Zhang, Li‑Na Zheng, Yong‑Qing Guo, Shu‑Zhen Yu, Jian‑Feng Wei, Xiao‑Lai Hou |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
medicine.medical_specialty Pathology CCR2 Chemokine hippocampus Ischemia Hippocampus chemokine receptor type 2 cerebral ischemia/reperfusion Chemokine receptor Immunology and Microbiology (miscellaneous) Internal medicine parasitic diseases medicine Receptor biology chemokine ligand 2 business.industry General Medicine Articles medicine.disease Real-time polymerase chain reaction Endocrinology biology.protein business Reperfusion injury |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | The aim of the present study was to determine the roles of the chemotactic factor, chemokine ligand 2 (CCL2), and its receptor, chemokine receptor type 2 (CCR2), in the hippocampus of rats with cerebral ischemia/reperfusion injury. In total, 24 Sprague-Dawley rats, weighting 250-300 g, were randomly divided into three groups (n=8): Sham-operated (C group), cerebral ischemia/reperfusion injury (I/R group) and propofol-intervention (P group) groups. The rats were sacrificed at 6 h after the ischemia/reperfusion surgery, and the brains were obtained to isolate the hippocampus. The mRNA expression levels of CCL2 and CCR2 in the hippocampus were analyzed by quantitative polymerase chain reaction, while the protein expression levels of CCL2 and CCR2 were determined by western blot analysis. The expression levels of CCL2 and CCR2 in the procerebrum were markedly elevated in the I/R and P groups at 6 h after the ischemia/reperfusion surgery when compared with the C group (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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