In vitro neutralization of antigenic variants of bluetongue virus is related to in vivo protection
| Autor: | Victoria C. Dean, Daniel R. Sundin, James O. Mecham, Kandi Mathiason DuBARD |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Serotype
medicine.drug_class Immunology Reoviridae Enzyme-Linked Immunosorbent Assay Monoclonal antibody Vaccines Attenuated Bluetongue Neutralization Virus Mice Antigen In vivo Neutralization Tests Virology medicine Animals Serotyping Mice Inbred BALB C Orbivirus biology Antibodies Monoclonal Viral Vaccines biology.organism_classification Precipitin Tests Molecular Medicine Electrophoresis Polyacrylamide Gel Bluetongue virus |
| Zdroj: | Viral immunology. 2(3) |
| ISSN: | 0882-8245 |
| Popis: | Neutralization resistant variants of bluetongue virus serotype 10 were selected with a neutralizing monoclonal antibody. Three variants were selected for further characterization. One of these variants was completely resistant to neutralization, while the other two variants showed intermediate resistance to neutralization as compared to the parent virus. The completely resistant variant failed to bind the selecting monoclonal antibody as determined by immune precipitation and enzyme linked immunosorbent assay; whereas, the other two variants bound antibody to a similar extent as the parent virus as determined by these tests. The ability of the variants to bind monoclonal antibody correlated with passive protection in the newborn mouse model. These results indicate that the ability of the virus to bind antibody is directly related to in vivo protection and that in vitro neutralization and in vivo protection are also related. |
| Databáze: | OpenAIRE |
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