Isolation and molecular characterization of Brazilian turkey reovirus from immunosuppressed young poults
| Autor: | Cilene Vidovix Táparo, Deriane Elias Gomes, Tereza C. Cardoso, Ana Carolina G. Rosa, Helena Lage Ferreira |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
CD4-Positive T-Lymphocytes
medicine.medical_specialty Turkeys Genotype PERUS medicine.medical_treatment Molecular Sequence Data Single group Biology CD8-Positive T-Lymphocytes Reoviridae Lymphoid Depletion Immunocompromised Host Viral Proteins Medical microbiology Bursa of Fabricius Virology Chlorocebus aethiops medicine Animals Cluster Analysis Antibody-Producing Cells Gene Vero Cells Phylogeny Poultry Diseases Sequence Homology Amino Acid Histocytochemistry Reverse Transcriptase Polymerase Chain Reaction Commercial Flock Brief Report Immunosuppression General Medicine Sequence Analysis DNA Isolation (microbiology) Reoviridae Infections Immunoglobulin M Vero cell Vero Cell RNA Viral Intense Damage CD8 Brazil Chicken Embryo Fibroblast |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Archives of Virology |
| Popis: | In this study, we investigated turkey reovirus (TReoV) in tissue samples from young birds, aged 15 days. RT-PCR for TReoV detected 3.3 % positive samples and TReoV was successfully isolated in Vero cells. Histological analysis of positive bursa of Fabricius (BF) revealed atrophied follicles and lymphocyte depletion. The number of CD8+, CD4+ and IgM+ cells was lower in infected BF. Phylogenetic analysis based on S3 gene showed that the Brazilian TReoV isolates clustered in a single group with 98-100 % similarity to TReoV strains circulating in the United States. This is the first indication that TReoV infection may be a contributing factor to immunosuppression in young birds. |
| Databáze: | OpenAIRE |
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