Linking of Bone Morphogenetic Protein-2 To Resorbable Fracture Plates for Enhancing Bone Healing
| Autor: | Sanghun Kim, Hoang Bang, Hausin Wong, Terry Y. Shibuya, Steven D. Feinberg, Eyiyemisi C. Damisah, Ashish Wadhwa, Abraham M. Panossian, Kevin Nguyen, P Ryan Camilon |
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| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Bone Morphogenetic Protein 2 Bone healing Bone morphogenetic protein Horseradish peroxidase Bone morphogenetic protein 2 Facial Bones Tissue Culture Techniques chemistry.chemical_compound Transforming Growth Factor beta Absorbable Implants medicine Humans Horseradish Peroxidase Carbodiimide Fracture Healing Bone growth Skull Fractures biology business.industry In vitro Otorhinolaryngology chemistry Bone Morphogenetic Proteins biology.protein Biophysics Interleukin-2 Alkaline phosphatase business Bone Plates |
| Zdroj: | The Laryngoscope. 115:2232-2237 |
| ISSN: | 0023-852X |
| DOI: | 10.1097/01.mlg.0000183692.27532.5b |
| Popis: | Objective: To test whether bone morphogenetic protein (BMP)-2 may be covalently linked to resorbable fracture repair plates using an ester-hydrolysis reaction and determining whether the linked compound can facilitate bone growth. Study Design: Laboratory in vitro experiments. Method: Resorbable fracture repair plates were partially hydrolyzed using varying concentrations of acid or base. This intermediate was then reacted with EDAC (1-ethyl-3[-3-dimethylamino propyl carbodiimide) to form an EDAC intermediate, which was then reacted with either horseradish peroxidase (HRP), interleukin (IL)-2, or BMP-2. Compound binding to the plate was confirmed by immunofluorescent staining. Confirmation of protein function was determined by the following assays: HRP's ability to cleave peroxide, IL-2's ability to stimulate lymphocytes, and BMP-2's ability to stimulate C3H10T1/2 cells to generate alkaline phosphatase. Results: Three compounds (HRP, IL-2, and BMP-2) were successfully linked to plates as confirmed by immunofluorescence staining or functional testing. Compounds demonstrated better covalent linking to plates under basic conditions. HRP, IL-2, and BMP-2 retained function after binding as measured by cleaved peroxide levels, lymphocytes proliferation, and alkaline phosphatase production. Conclusions: Covalent linking of compounds such as HRP, IL-2, and BMP-2 to resorbable plates is possible and represents a novel protein delivery technique. BMP-2 covalently linked to resorbable plates may be used to facilitate bone healing. Covalent linking of compounds to plates represents a novel method for delivering concentrated levels of growth factors to a specific site and potentially extending their half-life. Further investigation into this application for bone healing may lead to quicker healing. |
| Databáze: | OpenAIRE |
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