Rapid Susceptibility Test for Mycobacterium tuberculosis to Isoniazid and Rifampin with Resazurin Method in Screw-Cap Tubes
| Autor: | Belma Durupinar, C. Cekic Cihan, Ahmet Yilmaz Coban, Alper Akgunes, Ebru Cetinkaya, Kemal Bilgin, Meltem Uzun |
|---|---|
| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Rok vydání: | 2006 |
| Předmět: |
isoniazid
Tuberculosis medicine.drug_class Antibiotics Antitubercular Agents Microbial Sensitivity Tests Drug resistance Biology Sensitivity and Specificity Microbiology Mycobacterium tuberculosis chemistry.chemical_compound Predictive Value of Tests resazurin Oxazines Tuberculosis Multidrug-Resistant Isoniazid medicine Humans False Positive Reactions heterocyclic compounds Pharmacology (medical) Tuberculosis Pulmonary Antibacterial agent Pharmacology Rifamycin Drug Resistance Microbial Resazurin biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification medicine.disease Infectious Diseases Xanthenes Oncology chemistry Indicators and Reagents Rifampin rifampin medicine.drug |
| Zdroj: | Journal of Chemotherapy. 18:140-143 |
| ISSN: | 1973-9478 1120-009X |
| DOI: | 10.1179/joc.2006.18.2.140 |
| Popis: | WOS: 000237683000003 PubMed: 16736881 Multi-drug resistant (MDR) Mycobacterium tuberculosis is still a serious public health problem all over the world. MDR tuberculosis (MDR-TB) caused by these strains has emerged within the last decade and rapid detection is critical for the effective treatment of patients. Recently, a resazurin microtiter assay plate for detecting MDR strains was developed. In this study, it was adapted to screw-cap tubes and the activity of isoniazid (INH) and rifampin (RIF) to 50 M. tuberculosis clinical isolates was tested by this method for the first time. Results were compared with the radiometric reference method for the susceptibility testing of M. tuberculosis complex. The results of both methods were in 100% and 96% agreement for RIF and INH, respectively. Specificity, sensitivity, positive predictive value and negative predictive value were 91.7%, 100%, 92.8% and 100% for INH, respectively. All of these values were 100% for RIF. Susceptibility testing results were obtained on the 8th day of incubation for 42 isolates and on the 9(th) day for the other eight strains. Our results indicate that this method is suitable for the early determination of INH and RIF resistance in developing countries because it is inexpensive, rapid and easy to perform. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|