Oral administration of coated PEDV-loaded microspheres elicited PEDV-specific immunity in weaned piglets
| Autor: | Yun Zhang, Li Chen, Chunyi Xue, Du Yunping, Zhifen Wen, Yongchang Cao, Zhichao Xu, Qingfeng Zhou, Yunyan Wu, Wei Li |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Saliva Swine Administration Oral Enzyme-Linked Immunosorbent Assay Microbiology 03 medical and health sciences Antigen Oral administration Chlorocebus aethiops Medicine Animals Vero Cells Feces General Veterinary General Immunology and Microbiology biology business.industry Porcine epidemic diarrhea virus Vaccination Public Health Environmental and Occupational Health biology.organism_classification Small intestine Microspheres 030104 developmental biology Infectious Diseases medicine.anatomical_structure biology.protein Microscopy Electron Scanning Molecular Medicine Female Antibody business |
| Zdroj: | Vaccine. 36(45) |
| ISSN: | 1873-2518 |
| Popis: | Porcine epidemic diarrhea virus (PEDV) infects pigs of all ages by invading villous epithelial cells of the small intestine causing severe diarrhea with high mortality rate in suckling piglets. Mucosal immunity is believed to play an important role in PEDV control and mucosal delivery of vaccines induces mucosal immunity more efficiently than parenteral vaccination. In this study, coated PEDV-loaded microspheres with the size range of 700-900 μm in diameter were developed by centrifugal granulation-fluidized bed coating and demonstrated as an effective oral delivery system to protect PEDV antigens against the complex gastrointestinal environment by detecting the live virus particles in microspheres after the simulated gastric fluid treatment and the PEDV RNA in fecal swabs collected from all weaned piglets (100%) orally inoculated with coated PEDV-loaded microspheres. Weaned piglets orally immunized with coated PEDV-loaded microspheres developed higher levels of PEDV-specific antibodies (IgG and IgA) in their sera and saliva than those negative control groups (p 0.001 or p 0.01). Furthermore, neutralization assays demonstrated that serum antibodies in coated PEDV-loaded microspheres groups could significantly inhibit virus infection in Vero cells, compared to PEDV only group (p 0.05). Overall, our results indicate that the coated PEDV-loaded microspheres might serve as an effective way to induce PEDV-specific mucosal immunity in pigs against PEDV. |
| Databáze: | OpenAIRE |
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