MCM-41 Nanoparticles for Brain Delivery: Better Choline-Esterase and Amyloid Formation Inhibition with Improved Kinetics
| Autor: | Ajit K. Patra, Ashok Kumar Sharma, Sarita Rani, Anuj K. Sharma, Umesh Gupta, Gaurav Mishra, Awesh K. Yadav, Monika Rana, Pawan K. Pandey, Gopal Kandasamy |
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| Rok vydání: | 2021 |
| Předmět: |
Kinetics
Biomedical Engineering 02 engineering and technology Mesoporous silica 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences In vitro 0104 chemical sciences Bioavailability Biomaterials chemistry.chemical_compound chemistry Zeta potential Biophysics Choline Lysozyme 0210 nano-technology Ex vivo |
| Zdroj: | ACS biomaterials scienceengineering. 4(8) |
| ISSN: | 2373-9878 |
| Popis: | The present study was aimed at delivering a low bioavailability drug, rivastigmine hydrogen tartrate (RTG), to the brain through its encapsulation in mesoporous silica nanoparticles (MSNs) and targeted to amyloid inhibition in the brain. MSNs were characterized for size, zeta potential, and drug entrapment using SEM, TEM, HR-TEM, FT-IR, and PXRD. Drug-loaded MSNs were assessed for in vitro release kinetics and ex vivo followed by animal studies. The average size of the prepared blank (MCM-41B) and drug-loaded MSNs (MCM-41L) was 114 ± 2.0 and 145 ± 0.4 nm with the zeta potential of approximately -43.5 ± 1.1 and -37.6 ± 1.4 mV, respectively. MCM-41L exhibited an average entrapment efficiency of 88%. In vitro release studies exhibited early surge followed by a sluggish persistent or constant release (biphasic pattern). Hemolytic studies proved that the developed MCM-41L NPs are less hemolytic compared to RTG. A reduced ThT fluorescence was observed with MCM-41L compared to MCM-41B and RTG in the amyloid inhibition studies. A significant ( |
| Databáze: | OpenAIRE |
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