PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1
| Autor: | Man Li, Zheng Xue, Li-Quan Zhou, Jie Wu, Peng Zhang, Xue-Min Xie, Wen-Tian Wang, Xing Wang, Xiang Lv, Bei-Bei Mao, De-Pei Liu, Jian Xiong, Wei Yu, Zhi-Guo Zhao, De-Long Hao |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Gene isoform
Transcriptional Activation Myeloid Transcription Genetic Immunology Gene Expression Promyelocytic Leukemia Protein Biochemistry Promyelocytic leukemia protein Erythroid Cells hemic and lymphatic diseases Coactivator medicine Humans Protein Isoforms GATA1 Transcription Factor Protein Interaction Domains and Motifs Gata-Binding Protein Genetics biology Tumor Suppressor Proteins Nuclear Proteins Acetylation Cell Differentiation Zinc Fingers Cell Biology Hematology Cell biology Haematopoiesis medicine.anatomical_structure biology.protein Trans-Activators Erythropoiesis K562 Cells E1A-Associated p300 Protein K562 cells Protein Binding Transcription Factors |
| Zdroj: | Blood. 123(2) |
| ISSN: | 1528-0020 |
| Popis: | Promyelocytic leukemia protein (PML) has been implicated as a participant in multiple cellular processes including senescence, apoptosis, proliferation, and differentiation. Studies of PML function in hematopoietic differentiation previously focused principally on its myeloid activities and also indicated that PML is involved in erythroid colony formation. However, the exact role that PML plays in erythropoiesis is essentially unknown. In this report, we found that PML4, a specific PML isoform expressed in erythroid cells, promotes endogenous erythroid genes expression in K562 and primary human erythroid cells. We show that the PML4 effect is GATA binding protein 1 (GATA-1) dependent using GATA-1 knockout/rescued G1E/G1E-ER4 cells. PML4, but not other detected PML isoforms, directly interacts with GATA-1 and can recruit it into PML nuclear bodies. Furthermore, PML4 facilitates GATA-1 trans-activation activity in an interaction-dependent manner. Finally, we present evidence that PML4 enhances GATA-1 occupancy within the globin gene cluster and stimulates cooperation between GATA-1 and its coactivator p300. These results demonstrate that PML4 is an important regulator of GATA-1 and participates in erythroid differention by enhancing GATA-1 trans-activation activity. |
| Databáze: | OpenAIRE |
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