The 1,4-naphthoquinone derivative from Pyrola rotundifolia activates AMPK phosphorylation in C2C12 myotubes
| Autor: | Anna B. Ravcheeva, Leonid R. Ptitsyn, Ilya V. Sklyar, Kenzo Nomura |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Muscle Fibers
Skeletal Enzyme Activators 1 4-Naphthoquinone AMP-Activated Protein Kinases Cell Line chemistry.chemical_compound Mice Drug Discovery Animals Phosphorylation Protein kinase A Pyrola rotundifolia Pyrola Pharmacology biology Dose-Response Relationship Drug Molecular Structure Myogenesis Plant Extracts AMPK General Medicine biology.organism_classification Naphthoquinone Biochemistry chemistry C2C12 Naphthoquinones |
| Zdroj: | Fitoterapia. 82(8) |
| ISSN: | 1873-6971 |
| Popis: | An aqueous ethanol extract of Pyrola rotundifolia L. induced AMP-activated protein kinase (AMPK) phosphorylation in C2C12 myotubes. The bioassay-guided fractionation of the extract led to the isolation a 2-methyl-7-hydroxymethyl-1,4-naphthoquinone, or a 7′-hydroxy-chimaphilin, which showed concentration-dependent AMPK phosphorylation activity at 2.5–20 μg/ml. At a concentration of 10 μg/ml (50 μM), an approximately four-fold increase in the AMPKα(Thr172) phosphorylation level was observed. The stimulatory effect of naphthoquinone on AMPK activity was comparable to that of known compounds found in natural sources that activate the AMPK signaling pathway. |
| Databáze: | OpenAIRE |
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